转基因黄芪毛状根中黄芪甲苷含量的HPLC-ELSD法测定  

Determination on Contents of Astragaloside Ⅳ in Hairy Roots of Transgenic Astragalus Membranacius by HPLC-ELSD

作　　者：王子艳[1,2] 杜旻[1] 吴晓俊[1] 周吉燕[1] 王峥涛[1] 胡之璧[1] 

机构地区：[1]上海中医药大学中药研究所,上海201203 [2]上海中药标准化研究中心,上海201203

出　　处：《上海中医药大学学报》2010年第5期85-87,共3页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划基金资助项目(2006BAI09B01);上海市自然科学基金资助项目(06ZR14081;08ZR1419000)

摘　　要：目的:测定转黄芪毛状根中黄芪甲苷含量。方法:利用HPLC-ELSD法测黄芪甲苷含量。结果:转透明颤菌血红蛋白基因(Vitreoscilla Haemoglobin gene,vgb)黄芪毛状根、非转基因黄芪毛状根和山西产黄芪药材黄芪甲苷含量差异大。结论:转vgb黄芪毛状根黄芪甲苷含量远远高于非转基因黄芪毛状根和黄芪药材。Objective：To determine the contents of Astragaloside Ⅳ in hairy roots of Vitreoscilla Haemoglobin gene （vgb） -transgenic Astragalus membranacius. Methods： HPLC-ELSD was used to determine the contents of astragaloside Ⅳ. Results： There was great difference on the contents of astragaloside Ⅳ in Radix Astragali from Shanxi province,the hairy roots of non-transgenic Astragalus membranacius and hairy roots of vgb-transgenic Astragalus membranacius. Conclusion： The contents of astragaloside Ⅳ in hairy roots of vgb-transgenic Astragalus membranacius were much higher than that in Radix Astragali from Shanxi province and the hairy roots of non-transgenic Astragalus membranacius.

关 键 词：黄芪 毛状根 黄芪甲苷 透明颤菌血红蛋白 转基因 

分 类 号：R284.1[医药卫生—中药学]