南海北部油气微生物勘查研究  

Oil and Gas Microbiological Exploration in Northern South China Sea

作　　者：王庆光[1,2,3] 徐瑞松[1] 王洁[4] 

机构地区：[1]中国科学院广州地球化学研究所边缘海地质重点实验室,广州510640 [2]中国科学院研究生院,北京100049 [3]广东水利电力职业技术学院水利工程系,广州510635 [4]华东师范大学河口海岸研究所,上海200062

出　　处：《地质科技情报》2010年第5期56-61,共6页Geological Science and Technology Information

基　　金：国家973计划"南海北部珠江口盆地形成演化及油气资源潜力"(2007CB41170501);中国科学院知识创新工程项目"南海北部油气田海表烃类生物地球化学效应特征研究"(KZCXZ-YW-203-2)

摘　　要：随着现代生物技术的发展,微生物勘探技术正走向成熟,成为一项经济有效的直接找油方法。探讨了油气微生物勘探指标的选择和甲烷氧化菌的研究方法,应用原位荧光杂交法分析了南海北部表层海水中甲烷氧化菌的丰度,结果发现每升海水中有3.59×104～7.45×106个甲烷氧化菌,并且海水中甲烷浓度与甲烷氧化菌丰度的相关性较高,相关系数达0.861,基于两个指标综合分析海洋油气藏的赋存可以提高指示精度。With the development of modern biological technology,microbial technology is maturing and becoming an economically effective method for petroleum exploration.The paper explores the choice of indicators in petroleum microbe exploration and the research methods of methane oxidation bacteria,and analyzes the abundance of methane-oxidizing bacteria in surface seawater applied to fluorescence in situ hybridization.The results show that there are about 3.59×104—7.45×106 cells in per liter seawater,and there exists higher relevance between methane concentration and methane-oxidizing bacteria abundance.The accuracy of instructions of the occurrence of marine oil and gas reservoirs can be improved with com-prehensive analysis of the two indicators.

关 键 词：南海北部 微生物 甲烷氧化菌 荧光原位杂交(FISH) 

分 类 号：TE132.1[石油与天然气工程—油气勘探]