芹菜素对小鼠精液参数影响的研究  

Apigenin affects semen parameters in male mice

作　　者：史晓蓉 刘舒瑜[2] 陈亚[2] 李福轮[2] 薛红丽[2] 党瑜慧[2] 李芝兰[2] 

机构地区：[1]兰州市城关区妇幼保健所,甘肃兰州730030 [2]兰州大学公共卫生学院,甘肃兰州730030

出　　处：《中华男科学杂志》2010年第9期778-782,共5页National Journal of Andrology

基　　金：甘肃省中青年科技基金计划(099RJYA003)~~

摘　　要：目的:探讨芹菜素对小鼠精液参数的影响。方法:成年健康SPF级昆明种雄性小鼠100只按体重随机分成5个剂量组(阴性对照组、溶剂对照组及芹菜素低、中和高剂量组),各剂量组又随机分成7 d和14 d处理组。每天定时依次灌胃,7 d处理组和14 d处理组分别连续灌胃7 d和14 d,在首次灌胃第35 d时处死小鼠,取材,应用计算机辅助精液分析技术(CASA)检测小鼠精子运动参数。结果:芹菜素7 d处理各剂量组精子运动参数及精子密度、a+b级精子百分率与阴性对照组比较差异无统计学意义;芹菜素14 d处理高剂量组平均路径速度(VAP)、直线速度(VSL)、前向性(STR)、摆动性(WOB)、b级精子所占比例及a+b级精子百分率低于阴性对照组,鞭打频率(BCF)高于阴性对照组,均有统计学意义(P<0.05)。结论:芹菜素对小鼠精子运动参数有一定的影响。Objective：To explore the effect of apigenin on semen parameters in male mice.Methods：Totally 100 healthy male mice of Kunming strain were randomly divided into 5 groups according to the body weight：negative control,solvent control,low-dose apigenin,median-dose apigenin and high-dose apigenin,the latter three groups given intragastric apigenin at a fixed time every day for 7 and 14 days.At 35 days after the first medication,all the mice were killed and detected for the sperm motion parameters by computer aided sperm analysis（CASA）.Results：There were no statistically significant differences in sperm motion parameters,density and motility between the negative control and the three apigenin groups after 7-day medication.At 14 days,the high-dose apigenin group showed remarkable decreases in average path velocity（VAP）,straight line velocity（VSL）,straightness（STR）,wobbliness（WOB）,the percentage of grade b sperm and sperm motility,and a significant increase in beat cross frequency（BCF） as compared with the negative control group（P〈0.05）.Conclusion：Apigenin affects sperm motility in male mice to a certain extent.

关 键 词：芹菜素 精液参数 灌胃 

分 类 号：R698.2[医药卫生—泌尿科学]