五味子酚对3T3-L1细胞氧化应激模型脂肪细胞因子表达的影响  

Antioxidative effect of schisanhenol on adipokine expression in 3T3-L1 adipocyte

作　　者：季振中[1] 代喆[1] 牛力[1] 黄艳[1] 徐焱成[1] 

机构地区：[1]武汉大学中南医院内分泌科,武汉430071

出　　处：《中国医师杂志》2010年第9期1153-1157,共5页Journal of Chinese Physician

基　　金：国家自然科学基金（30871188）

摘　　要：目的 研究五味子酚（schisanhenol,SAL）对3T3-L1细胞脂肪因子表达的影响及其机制.方法 体外培养并诱导分化3T3-L1细胞,制作氧化应激模型,用不同浓度和作用时间的五味子酚干预,观察脂肪因子表达的变化.结果 （1）脂联素、瘦素、抵抗素和内脂素的表达随着氧化应激的程度增高递减,其中对瘦素的抑制效应最明显（25 mU/ml葡萄糖激酶组VS空白组,t=7.29,P＜0.01）;（2）4种脂肪因子的表达随着五味子酚干预浓度增加而增加（脂联素t=6.31,P＜0.01;瘦素t=5.92,P＜0.01;抵抗素t=3.77,P＜0.05;内脂素t=3.63,P＜0.05）;（3）随着五味子酚作用时间的延长,4种脂肪因子的表达均经历了先下降后上升的过程,且五味子酚对脂肪细胞因子表达的影响与氧化应激状态的改变平行.结论 五味子酚可通过抗氧化应激作用调节脂肪因子的表达,可能在五味子改善糖尿病症状过程中起到重要作用.Objective To explore the effect of schisanhenol on adipokine expression in 3T3-L1 adipocyte and its related mechanism. Methods 3T3-L1 adipocyte was cultured in vitro and induced to differentiation and maturity. Glucokinase was added to culture medium to make an oxidative model. The expression of adipocytokines were detected under the circumstance of different doses and at different time points of schisanhenol. Results The expression of adiponectin, leptin, resistin and visfatin were decreased with the increase of glucokinase concentration. Concentration-dependent inhibition effect was most obvious in leptin （25 mU/ml Glucokinase vs Blank group, t =7.29, P〈0.01）. With pretreatment of oxidative stress, the adipocytokines increased as the doses of schisanhenol increased （t=6.31,P〈0.01 in adiponectin;t=5.92, P〈0.01 in leptin; t=3.77, P〈0.05 in resistin; t=3.63,P〈0.05 in visfatin）. With the extension of schisanhenol effect, the expression of four adipokines showed the process of first decrease-then increase＇. The effects of schisanhenol on adipokines were parallel with the alteration of oxidative stress. Conclusions Schisanhenol increased adipocytokines expression in 3T3-L1 adipocyte by reducing oxidative stress, and the increase of leptin and adiponectin were most obvious, which indicated that schisanhenol could play a role in the treatment of diabetes by Chinese herb wuweizi.

关 键 词：五味子/药理学 氧化性应激/药物作用 脂肪因子类/代谢 

分 类 号：R28[医药卫生—中药学]