纳金在包埋前免疫电镜中的应用  

An Application of Nanogold to Preembedding Immunoelection Microscope

作　　者：张亦农[1] 李和[1] 

机构地区：[1]同济医科大学组织学与胚胎学教研室

出　　处：《解剖科学进展》1999年第2期166-170,共5页Progress of Anatomical Sciences

摘　　要：本文介绍纳金（ｎａｎｏｇｏｌｄ，一种直径仅为１．４ｎｍ的超微金化合物）在包埋前单标和双标免疫电镜中的应用。电镜下观察到，以纳金标记的第７型代谢性谷氨酸受体（ｍＧｌｕＲ７）在大鼠脊髓后角浅层内定位于轴突终末，包括一些Ｉ－Ｂ４（一种从Ｇｒｉｆｏｎｉｇｓｉｍｐｌｉｃｉｆｏｌｉｇ种子中得到的凝集素）标记的一级传入Ｃ纤维终末内的突触前膜上；在大鼠疑核内，μ阿片受体（ＭＯＲ）主要存在于树突内的非突触部位。此外，我们还观察到，Ｐ物质（ＳＰ）与Ｉ－Ｂ４结合位点共存于一级传入Ｃ纤维中枢突终末内。实验证明，纳金标记的ＩｇＧ或其Ｆａｂ′片段是一种具有良好渗透性的免疫金探针，应用这种探针的包埋前免疫电镜术具有敏感性高、背景低、分辨率高等优点，尤其适于受体超微定位。e report an application of nanogold, an extremely small gold compound (1.4nm diameter), to singleor doublepreembedding immunoelectron microscopy. Under electron microscope, it was obesrved that, in the superficial laminae of the spinal dorsal horn of the rat, a metabotropic glutamate receptor, mGluR7, was localized in the presynaptic membranes of the axonal terminals some of which were the terminals of primary afferent C fibers labelled with isolectin IB4 from the seeds of Griffonia simplicifolia, and that opioid receptor (MOR) was mainly distributed in nonsynaptic sites of dendrites in rat ambiguous nucleus. It was also found that substance P(SP) was colocalized with IB4 binding afferent C fiber. From the practice it is demonstrated that IgG or its Fab fragment conjugated with nanogold is an immunogold probe which has the advantages of low background, high penetration, superior sensitivity and high resolutionan and sutis ultrastructural localization of receptors in particular when it is used in preembedding immunoelectron microscopy.

关 键 词：纳金 免疫电镜 受体 

分 类 号：Q－336[生物学]