异氟醚和七氟醚及地氟醚对大鼠肺微血管内皮细胞ICAM-1和VCAM-1表达的影响  

Effects of isoflurane,sevoflurane and desflurane on expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells

作　　者：罗行[1] 缪长虹[1] 戈宝学[2] 蒋豪[1] 

机构地区：[1]复旦大学附属中山医院麻醉科,上海200032 [2]中科院上海健康研究所,上海200025

出　　处：《浙江大学学报（医学版）》2010年第5期464-469,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(30872430)

摘　　要：目的:观察异氟醚、七氟醚和地氟醚对大鼠肺微血管内皮细胞(RLMVECs)ICAM-1和VCAM-1表达的影响,探讨吸入性麻醉药物保护肺泡毛细血管屏障功能的可能机制。方法:0.7MAC、1.0 MAC、2.0 MAC的异氟醚、七氟醚和地氟醚处理经LPS(100 ng/ml)诱导6 h的RLMVECs,采用Western blot检测ICAM-1和VCAM-1的蛋白表达量,RT-PCR半定量法检测ICAM-1水平。结果:1.0 MAC的异氟醚对LPS诱导的RLMVECs ICAM-1的表达有上调作用(P<0.05);1.0 MAC的七氟醚、地氟醚对LPS诱导的RLMVECs ICAM-1的表达有下调作用(P<0.05);2.0 MAC的地氟醚对RLMVECs ICAM-1的表达有上调作用(P<0.05)。不同吸入麻醉药物对LPS诱导的RLMVECs VCAM-1的表达作用随浓度上升而下调,呈剂量依赖性。结论:1.0 MAC的七氟醚和地氟醚对LPS诱导的RLMVECs ICAM-1的表达有下调作用,这可能是其在急性肺损伤时减少中性粒细胞募集、保护肺泡毛细血管屏障功能的分子学机制。Objective： To investigate the effect of isoflurane,sevoflurane and desflurane on the expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells（RLMVECs）.Methods： Cultured LPS-treated RLMVECs were exposed to 0.7,1.0 or 2.0 minimum alveolar concentration（MAC） iosoflurane,sevoflurane or desflurane in 6 h.The protein expression of ICAM-1 and VCAM-1 was determined by Western blot analysis.The expression of ICAM-1 mRNA was detected by reverse-transcription polymerase chain reaction（RT-PCR）.Results： Isoflurane at concentration of 1.0 MAC up-regulated the expression of ICAM-1 in LPS-induced RLMVECs（P0.05）;while same concentrations of sevoflurane and desflurane down-regulated the expression of ICAM-1（P0.05）.Desflurane at concentration of 2.0 MAC up-regulated the expression of ICAM-1 in non-LPS-induced RLMVECs.All the volatile anesthetics down-regulated the expression of VCAM-1 in a dose-dependent manner.Conclusion： Compared to isoflurane,1.0 MAC sevoflurane and desflurane down-regulate the expression of ICAM-1,which may be the molecule mechanism of their protective effect in acute lung injury.

关 键 词：脂多糖类 毛细血管 内皮 血管/细胞学 麻醉药 吸入/药理学 异氟醚/投药和剂量 醚类/投药和剂量 胞间粘附分子-1 血管细胞粘附分子-1 

分 类 号：R965[医药卫生—药理学]