体外循环心内直视术对外周血单个核细胞蛋白质表达谱的作用  

A proteomic study of peripheral blood mononuclear cells in patients undergoing cardiopulmonary bypass

作　　者：杨艳琴[1] 唐靖[1] 古妙宁[1] 郭远波[1] 赵振龙[1] 陈晔明[1] 陈雯婷[1] 

机构地区：[1]南方医科大学附属南方医院麻醉科,广东广州510515

出　　处：《浙江大学学报（医学版）》2010年第5期470-476,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助(30872431)

摘　　要：目的:观察体外循环(CPB)心内直视术患者外周血单个核细胞蛋白质表达谱的变化。方法:11例体外循环心内直视术患者,分别于体外循环前(T0)、体外循环主动脉开放后1 h(T1)和术毕(T2)3个时点抽取动脉血,分离外周血单个核细胞(PBMCs),采用双向电泳和质谱方法比较、分析3个时间点差异蛋白的表达,并采用W estern blot进行验证。结果:双向电泳和质谱鉴定出12个差异蛋白,其中S100A9表达量于CPB后1 h达到高峰,手术结束时开始下降,但未回复到术前水平(P<0.05)。结论:体外循环能上调外周血单个核细胞S100A9等蛋白的表达。Objective： To explore the effect of cardiopulmonary bypass（CPB） on the profile of protein expression in peripheral blood mononuclear cells（PBMCs）.Methods： Eleven patients undergoing cardiac surgery under cardiopulmonary bypass were enrolled in the study.Peripheral blood samples were collected before CPB（T0）,1 h after CPB（T1） and at the end of operation（T2）,and PBMCs were obtained by gradient centrifugation.The profile of protein expression was analyzed using 2-D gel electrophoresis（2-DE） and mass spectrometry.The candidate proteins were further identified by Western blotting.Results： Compared to protein profile at T0,12 protein spots were identified to be up-regulated in PBMCs at T1（P0.05）,among which S100A9 reached the peak level at T1 and decreased after operation,but not returned to its initial level.Conclusion： Results indicate that 12 proteins are likely to be involved in CPB,however,their roles need to be elucidated.

关 键 词：体外循环 S100蛋白质类/代谢 炎症 单核细胞 电泳 凝胶 双向 

分 类 号：R654.1[医药卫生—外科学]