肠道病毒B组山东地方株的基因型分布  

Genotype Distribution of Enterovirus Group B Isolated in Shandong Province,China

作　　者：王海岩[1] 刘昆[2] 陶泽新[1] 李岩[1] 刘桂芳[1] 刘尧[1] 冯蕾[1] 樊庆莹[3] 杨赫[3] 宋立志[1] 徐爱强[1,3] 

机构地区：[1]山东省疾病预防控制中心山东省传染病预防控制重点实验室,济南250014 [2]山东大学齐鲁医院,济南250012 [3]山东大学公共卫生学院,济南250012

出　　处：《病毒学报》2010年第5期357-362,共6页Chinese Journal of Virology

基　　金：国家科技重大专项<病毒性传染病病原谱流行规律及变异研究>课题(2009ZX10004-202);山东省医药卫生重点项目计划课题(2007HZ090)

摘　　要：为明确源自急性弛缓性麻痹（AFP）病例的HEV-B组病毒山东地方株的基因型分布,探讨其优势基因型的变迁与疾病暴发之间的关系,本研究对山东省1994年~2008年AFP监测系统分离到的HEV-B组病毒进行了VP1区核酸扩增和序列测定。序列测定结果显示HEV-B山东地方株共包括29种基因型,其中CVA 1种（CVA9）,CVB 5种（CVB1~5）,ECHO 20种以及新型肠道病毒EV73、75、97。其中ECHO11、CVB3、ECHO6、ECHO14、ECHO25是AFP监测系统中最常分离到的B组病毒。同源性比较显示,相同血清型HEV-B山东地方株型内核苷酸同源性最小75.4%,最大99.6%,与原型株核苷酸同源性最小73.8%,最大85.2%,但氨基酸变异不大。研究表明,不同基因型病毒具有不同的时间循环模式,相同基因型毒株内部根据其遗传距离的远近又可划分为不同的基因亚型,从而帮助确定HEV的传播途径和传播范围。In order to explore the genotype distribution of human enterovirus group B（HEV-B） in Shandong Province and to study the correlation between HEV-B serotypes and disease outbreaks,we sequenced and analyzed the entire VP1 coding region of HEV-B isolated from acute flaccid parolysis（AFP） system and disease outbreaks in Shandong province during 1998-2008.All together twenty nine HEV-B serotypes were identified,including twenty Echovirus（ECHO） serotypes,five Coxsackievirus B（CVB1-5） serotypes,one Coxsackievirus A9（CVA9） serotype,and newer enteroviruses EV73,EV75,and EV97.E11,CVB3,E6,E14 and E25 were the five frequently isolated serotypes from AFP surveillance system.CVB3,CVB5 and ECHO30 were the major causative agent of aseptic meningitis in Shandong province.Comparison of nucleotide sequence homology showed 75.4%-99.6% inter-typic identities within Shandong strains,and 73.8%-85.2% identities with prototype strains.Amino acid sequence comparison showed the differentiation was not much.Our research showed different serotypes possessed distinct time-cycling pattern,and different sub-genotypes could be further classified according to the inter-typic genetic distance.Thereby the route and range of transmission of HEV could be determined.

关 键 词：肠道病毒 急性弛缓性麻痹(AFP) 无菌性脑膜炎 基因型 

分 类 号：R373.2[医药卫生—病原生物学]