焦磷酸测序技术在禽流感病毒金刚烷胺耐药性检测中的应用  

Detection of Molecular Markers of Amantadine Resistance in Avian Influenza Viruses by Pyrosequencing

作　　者：刘华雷[1] 吕艳[1] 郑东霞[1] 赵云玲[1] 孙承英[1] 张维[1] 李金明[1] 王志亮[1] 

机构地区：[1]中国动物卫生与流行病学中心,青岛266032

出　　处：《病毒学报》2010年第5期392-395,共4页Chinese Journal of Virology

摘　　要：流感病毒M2蛋白五个关键位点氨基酸残基(第26、27、30、31和34位)中的任何一个发生突变都会导致抗流感病毒药物中金刚烷胺抗药性的产生。本研究利用焦磷酸测序技术对94株不同亚型禽流感病毒金刚烷胺耐药性分子决定区进行了鉴定,并进行抗药性分析。结果表明94株禽流感病毒中有81株M2基因存在金刚烷胺耐药性的分子标签,其余的13株根据分子标签判断为对金刚烷胺敏感。耐药性的分子标记存在V27I和S31N两种突变形式,其中绝大多数为S31N。Mutation in any of five key amino acid residues（at positions 26,27,30,31 and 34） within the M2 protein of influenza A viruses leads to resistance against the amantodine class of anti-influenza drugs.In this study,a pyrosequencing method was described to rapidly detect established five molecular markers of resistance to M2 blockers,amantadine.The residues L26,V27,A30,S31 and G34 in the M2 protein were targeted for pyrosequencing,and 94 avian influenza viruses were used to perform the amantadine resistance analysis.Our results showed that most of avian influenza viruses were amantadine resistant,Mutations V27I and S31N were founded in these isolates.

关 键 词：禽流感病毒 焦磷酸测序 抗药性分析 

分 类 号：S852.659.5[农业科学—基础兽医学]