炭疽灭活和裂解芽胞抗原免疫家兔后血清中的IgG抗体应答  

The sera IgG response of rabbits after immunization with anthrax killed and split vaccines

作　　者：韩少波[1] 尤明强[1] 惠一鸣[1] 傅林锋[1] 胡丽娜[1] 魏东[2] 王秉翔[1] 

机构地区：[1]兰州生物制品研究所,兰州730046 [2]中国药品生物制品检定所,北京100050

出　　处：《微生物学免疫学进展》2010年第3期9-12,共4页Progress In Microbiology and Immunology

摘　　要：炭疽杆菌芽胞在炭疽免疫中发挥基本作用。实验中以炭疽活芽胞疫苗为原形,建立了制备灭活和裂解炭疽芽胞的方法,研究了各种灭活和裂解炭疽芽胞疫苗不同浓度、不同剂次免疫家兔的抗芽胞和毒素IgG应答,总结分析了各种灭活和裂解炭疽芽胞疫苗用于新疫苗成分之一的可能性。甲醛灭活炭疽芽胞疫苗设芽胞浓度2.5×108剂量组、5×108剂量组、1×109剂量组,于0、4、8周时3次免疫。在3剂免疫后血清抗炭疽芽胞IgG水平持续升高,首次免疫后4、8、12周时家兔血清中抗芽胞IgG几何平均滴度可达到600～16000。裂解炭疽芽胞疫苗的制备和动物免疫中,只采取了2.5×108芽胞浓度,两剂免疫,免疫时间为0、4周。在首次免疫后4、8、12周时家兔血清中抗芽胞IgG几何平均滴度分别为362、776和388。各时间点采集的家兔血清未能测出或只测出极微量的抗炭疽毒素IgG。通过上述研究认为,以裂解炭疽芽胞抗原作为炭疽疫苗成分之一,其抗原性和免疫原性是适宜的;免疫剂量可以设定为2.5×108芽胞浓度上下;免疫次数可定为2剂间隔1个月。Anthrax spores make an essential contribution to vaccine efficacy.The method for manufacturing killed and split B.anthracis spores were estabolished referring to the preparation of the human anthrax live attenuated vaccine.Anti-spore and anti-toxin sera IgG responses of rabbits were detected after immunizing with the vaccines in different concentration and dosages.Three groups of rabbits were immunized with 2.5×10^8,5×10^8 and 1×10^9 of the formalin killed B.anthracis spore respectively for three times at 0,4 and 8 weeks.The tested results showed that the anti-spore sera IgG titers of rabbits immunized 3 doses raise constantly,the geometric mean titers can reach 600 to 16000 at week 4,8 and 12 after the first dose.The IgG antibody responses of rabbits immunized with two doses of 2.5×10^8 spores were detected at 4,8 and 12 weeks after the first dose,the anti-spore IgG geometric mean titers are 752,724 and 322 respectively.None or very low anti-toxin IgG titers can be detected for both killed and split anthrax vaccines at each set time.In conclusion,in view of the above-mentioned results,the split B.anthracis spores antigen can be as a component of the anthrax vaccine,in the immune-schedule two doses of approximate 2.5×10^8 spores can be inoculated in one month interval.

关 键 词：炭疽杆菌 炭疽疫苗 炭疽芽胞抗原 血清IgG水平 

分 类 号：R392[医药卫生—免疫学]