甲型H1N1流感病毒佐剂疫苗小鼠免疫效果  

Study on immunity effect of influenza A (H1N1) virus vaccine with adjuvant in mice

作　　者：常军亮[1] 陈维金[1] 李春晖[2] 吴业红[1] 王莹[1] 徐军[1] 王伟善[1] 郭立君[1] 郭凤云 

机构地区：[1]长春生物制品研究所,长春130062 [2]吉林大学第二临床医院,长春130041

出　　处：《微生物学免疫学进展》2010年第3期16-18,共3页Progress In Microbiology and Immunology

摘　　要：为了探讨甲型H1N1流感病毒氢氧化铝佐剂疫苗对小鼠的免疫作用及对小鼠繁殖性能的影响,以不同剂量、不同免疫程序免疫小鼠后定期采血;用血凝抑制(HI)方法检测血清H1N1流感病毒HI抗体滴度,观察H1N1流感病毒佐剂疫苗对小鼠受孕、产仔、哺乳的影响;比较孕鼠及非孕鼠的抗体滴度,免疫后孕鼠所产仔鼠的体重及H1N1胎传抗体水平。结果显示,以0.5μg组开始的不同剂量、不同免疫程序均可使小鼠产生90倍以上水平的H1N1流感病毒抗体;免疫后的小鼠不影响受孕、产仔及哺乳;仔鼠保护性抗体可持续1个月以上。H1N1流感病毒佐剂疫苗是一种高免疫原性的制剂,用低剂量免疫,即可产生90倍以上持续时间较长的保护性抗体。这种佐剂疫苗对小鼠的繁殖性能无明显影响,免疫产生的抗体经胎盘可垂直传递给仔鼠。To investigate the immunogenicity of influenza A H1N1 virus vaccine with Al（OH）3 adjuvant and the influence of this vaccine on the productivity of mice.The mice were immunized with influenza A H1N1 virus vaccine with Al（OH）3 adjuvant in different schedules and dose,and blood samples were collected at the scheduled time.The HI antibody titers against influenza A H1N1 virus were tested,the pregnancy,birth and lactation status of mice after vaccinating were observed.The results showed that begin with 0.5μg,the influenza A H1N1 vaccine can induce the H1 antibody titers raising 90 times in the conditions of different doses and immunization schedules.The protective antibody of newborn mice can maintain for one month.The pregnancy,birth and lactation of the immunized mice can not affected by vaccine inoculation.

关 键 词：甲型H1N1流感病毒 佐剂疫苗 免疫原性 繁殖性能 

分 类 号：R392.1[医药卫生—免疫学]