急性心肌梗塞患者外周血中循环内皮细胞含量的改变及凋亡的检测  

Changes in the Level of Circulationg Endothelial Cells in Acute Myocardial Infarction Patients and the Detection of Its Appotosis

作　　者：迟喧[1] 李宏伟[1] 肖林生 修瑞娟[1] 

机构地区：[1]中国医学科学院,中国协和医科大学微循环研究所,北京市100005

出　　处：《中国微循环》1999年第2期102-105,共4页Journal of Chinese Microcirculation

摘　　要：目的应用我所新建立的循环内皮细胞（Ｃｉｒｃｕｌａｔｉｎｇ Ｅｎｄｏｔｈｅｌｉａｌ Ｃｅｌｌｓ，ＣＥＣ）的分离计数方法计数急性心肌梗塞患者外周血中循环内皮细胞含量的改变并检测其凋亡。方法Ｐｅｒｃｏｌｌ密度梯度离心后，将其先后标记以鼠抗人ＣＤ３１抗体，ＦＩＴＣ－羊抗鼠ＩｇＧ及碘化丙啶。应用流式细胞伙计数ＣＤ３１阳性细胞的百分比，并通过检测ＣＥＣ的ＤＮＡ倍体含量检测其凋亡状态。结果急性心肌梗塞组（ｎ＝１６）与正常对照组（ｎ＝１６）的 ＣＥＣ分别为 ６２．９±２４．５／ｍｌ及 ２９． ９±９．１／ｍｌ全血，两者有非常显著性差异（Ｐ<０．０００１）。两组均为双倍体ＤＮＡ，无亚二倍体峰的出现，说明无细胞凋亡的发生。结论此方法克服了以往ＣＥＣ研究方法的缺点，提高了特异性及回收率，使ＣＥＣ真正成为来自活体的特异的且直接反映血管损伤的量化指示物。Objective The aim of this study is to count CEC in peripheral blood of acute myocardial infarction (AMI) patients and to detect its apoptotic state,using FACS analysis. Methods The peripheral blood was Percoll density gradient centrifuged and analyzed with flow cytometry,using CD31 monoclonal antibody and propidium iodide.Results CEC counting is 62.9?4.5/ml and 29. 9?. 1/ml whole blood in AMI patients (n=16) and age-matched controls (n=16) respectively (P<0.0001 ). There were no signs of apoptosis in both groups. Conclusion There was significant increase in CEC level in AMI patients,indicating that endothelial injury in AMI patients occurred significantly.Our result proved that FACS analysis is efficient in counting and identifying the functional status of CEC in various cardiovascular diseases.

关 键 词：心肌梗塞 循环内皮细胞 细胞凋亡 

分 类 号：R542.220.2[医药卫生—心血管疾病]