RP-HPLC同时测定天麻中对羟基苯甲酸和4,4′-二羟基二苄砜的含量  

RP-HPLC simultaneous determination of p-hydroxybenzoic acid and 4,4'-dihydroxybenzyl sulfone in the rhizome of Gastrodia elata Bl.

作　　者：闫宝庆[1] 张晖芬[1] 逄楠楠[1] 陈晓辉[1] 于治国[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2010年第9期1729-1731,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立天麻中对羟基苯甲酸和4,4′-二羟基二苄砜的含量测定方法。方法:采用HPLC法,使用KromasilC18(250mm×4.6mm,5μm)色谱柱,以甲醇-0.1%醋酸水溶液为流动相,梯度洗脱,流速1.0mL.min-1,检测波长240nm,柱温35℃。结果:对羟基苯甲酸和4,4′-二羟基二苄砜进样浓度分别在0.421～8.41μg·mL-1(r=0.9992)和0.650～13.0μg.mL-1(r=0.9999)范围内与峰面积线性关系良好;平均加样回收率(n=9)分别为98.2%和98.7%。结论:采用HPLC法测定天麻中对羟基苯甲酸和4,4′-二羟基二苄砜的含量方法准确可靠,可用于天麻的质量控制。Objective：To establish a reversed-phase HPLC method for simultaneous determination of p-hydroxybenzoic acid and 4,4＇-dihydroxybenzyl sulfone in the rhizome of Gastrodia elata Bl..Methods：A Kromasil C18（250 mm×4.6 mm,5 μm） column was adopted with a methanol-water-0.1% acetic acid gradient elution system.The elution were detected at 240 nm,the flow rate was 1.0 mL·min-1 and the column temperature was 35 ℃.Results：The linear range of p-hydroxybenzoic acid and 4,4＇-dihydroxybenzyl sulfone were 0.421-8.41 μg·mL-1（r=0.9992）,0.650-13.0 μg·mL-1（r=0.9999）,respectively.The average recoveries（n=9） of the two components were 98.2%,98.7%,respectively.Conclusion：The method is accurate,sensitive and reliable for determination of p-hydroxybenzoic acid and 4,4＇-dihydroxybenzyl sulfone in the rhizome of G. elata. And this method can be used for the quality control research of Gastrodia elata Bl..

关 键 词：天麻 RP-HPLC 对羟基苯甲酸 4 4′-二羟基二苄砜 

分 类 号：R917[医药卫生—药物分析学]