HPLC法测定布渣叶中牡荆苷和异鼠李素-3-O-β-D-芸香糖苷的含量  

HPLC determination of vitexin and isorhamnetin-3-O-β-D-rutinoside in Microcos paniculata L.

作　　者：李坤平[1,2] 潘天玲[1] 高崇凯[1] 李卫民[2] 

机构地区：[1]广东药学院,广州510006 [2]广州中医药大学,广州510006

出　　处：《药物分析杂志》2010年第9期1746-1749,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法同时测定布渣叶中牡荆苷和异鼠李素-3-O-β-D-芸香糖苷含量。方法:采用KromasilC18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水溶液,二元梯度洗脱,流速1.0mL.min-1,检测波长276nm,柱温35℃。结果:牡荆苷和异鼠李素-3-O-β-D-芸香糖苷的线性范围分别为0.0412～0.412μg(r=0.9992)和0.0424～0.424μg(r=0.9991);平均回收率(n=9)分别为96.2%和96.0%。结论:该方法简便、准确,可用于布渣叶中牡荆苷和异鼠李素-3-O-β-D-芸香糖苷含量的同时测定。Objective：To develop an RP-HPLC method for the simultaneous analysis of vitexin and isorhamnetin-3-O-β-D-rutinoside in Microcos paniculata L.. Methods：HPLC determination was performed on a Kromasil C18 column（250 mm×4.6 mm,5 μm）with a binary elution of methanol-0.1 % phosphoric acid aqueous solution as mobile phase and detected at 276 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 35 ℃. Results：The calibration curves were linear in the ranges of 0.0412-0.412 μg（r=0.9992）and 0.0424-0.424 μg（r=0.9991）for vitexin and isorhamnetin-3-O-β-D-rutinoside,and the average recoveries（n=9）of vitexin and isorhamnetin-3-O-β-D-rutinoside were 96.2% and 96.0%,respectively.Conclusion：This method is simple and accurate,which can be used for the simultaneous determination of vitexin and isorhamnetin-3-O-β-D-rutinoside in Microcos paniculata.

关 键 词：布渣叶 高效液相色谱法 牡荆苷 异鼠李素-3-O-β-D-芸香糖苷 

分 类 号：R917[医药卫生—药物分析学]