陕西产秦艽质量变异与遗传多样性研究  

Studies on quality variance and genetic diversity of Gentianae Macrophyllae Radix from different habitats in Shaanxi Province

作　　者：石张燕[1,2,3] 陈千良[1,2,3] 赵宇玮[1,2] 孙文基[2,3] 赵桂仿[1,2] 

机构地区：[1]西北大学西部资源生物与现代生物技术教育部重点实验室,陕西西安710069 [2]西北大学生命科学学院,陕西西安710069 [3]西北大学陕西省生物医药重点实验室,陕西西安710069

出　　处：《中草药》2010年第10期1705-1709,共5页Chinese Traditional and Herbal Drugs

基　　金：科技部"重大新药创制"科技重大专项(2008ZX09101-2-106);陕西省教育厅专项科研计划项目(06JK178);陕西省自然科学基金资助项目(SJ08-ZT02-4)

摘　　要：目的研究陕西省不同产地秦艽的质量差异,对其遗传多样性进行检测,探讨药材质量和其遗传基础的关系。方法采用HPLC法测定不同产区秦艽药材中的指标成分,进行HPLC指纹图谱分析,计算并比较相似度。依据指纹图谱中的共有峰特征,对不同药材样品进行聚类分析。对药材中提取的总DNA进行RAPD分析,计算遗传距离并聚类。结果陕西省不同产区秦艽药材的质量具有一定的差异,不同产区秦艽药材也表现出一定的遗传多样性。陕西省不同产区的秦艽药材质量变异与遗传多样性之间不具有明显的相关性,而表现出与产地的相关性。结论产地对陕西秦艽药材质量的影响更为重要,提示道地秦艽质量的形成可能属于生境主导型。Objective The quality variance of Gentianae Macrophyllae Radix from different habitats of Shaanxi Province was studied,the genetic diversity was detected,and the relationship between quality and inheritance was discussed.Methods Using HPLC to measure effective components of Gentianae Macrophyllae Radix from different habitats,analyze HPLC fingerprint,and calculate and compare the similarity.Based on traits of common peaks in fingerprint,different samples of Gentianae Macrophyllae Radix were clustered.The total DNA from crude medicinal materials was analyzed by RAPD,the genetic distance was calculated and different samples were clustered at the base of the genetic distance.Results There were quality variance and genetic diversity at a certain degree with Gentianae Macrophyllae Radix from different habitats of Shaanxi Province.There was no apparent correlation between quality variance and genetic diversity of Gentianae Macrophyllae Radix from different habitats of Shaanxi Province,but there was correlation between quality variance and habitats.Conclusion The habitats are more important for the quality of Gentianae Macrophyllae Radix in Shaanxi Province.This suggests that the quality of Gentianae Macrophyllae Radix is mainly affected by the environment.

关 键 词：秦艽 质量变异 遗传多样性 

分 类 号：R284.1[医药卫生—中药学]