金丝桃苷对小鼠四氯化碳肝损伤的保护作用  

Protective effect of hyperin on CCl_4-induced hepatic injury in mice

作　　者：孔华丽[1,2] 胡克章[3,2] 杨新波[4] 黄正明[1] 

机构地区：[1]解放军第309医院药剂科,北京100091 [2]解放军第302医院药剂科,北京100039 [3]内江市第一人民医院药剂科,四川641000 [4]解放军总医院老年病研究所药理研究室,北京100085

出　　处：《中国新药杂志》2010年第19期1794-1797,1820,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30572350);国家中医药管理局“十五”新药基金(DIX005A)

摘　　要：目的:观察金丝桃苷(hyperin,Hyp)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将180只雌雄小鼠分为3批进行实验。每批小鼠随机分为正常对照组,CCl4模型对照组,联苯双酯阳性对照组,Hyp高、中、低(50,25和12.5 mg.kg-1)3个剂量组。各给药组均连续给药7 d,末次给药1 h后,给药组与模型组均ip 0.1%CCl4油剂(0.1 mL/10 g体重)。中毒小鼠16 h后眼球取血,测定血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)水平,并迅速处死小鼠取肝,检测肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力,并行肝切片镜检观察各组肝脏的病理变化。结果:CCl4模型组小鼠肝功能明显异常,MDA含量明显升高,SOD活力明显下降。Hyp组能明显改善CCl4中毒小鼠的肝功能,使其ALT和AST显著降低,高、中、低剂量组降酶率分别为89.6%,86.5%和78.3%;肝组织中SOD活力明显升高,MDA含量降低;CCl4模型组小鼠肝脏损伤严重,Hyp高剂量组的肝病理改变明显轻于CCl4模型组。结论:Hyp对CCl4急性肝损伤小鼠有较好的保肝降酶作用。Objective： To observe the protective effect of hyperin（Hyp） on acute liver injury induced by CCl4 in mice.Methods： A total of 180 mice were randomly divided into 3 groups,and acute liver injury was induced by ip 0.1% CCl4（0.1 mL/10 g）.Mice were treated by bifendate or Hyp（12.5,25 and 50 mg·kg-1） for 7 days.Serum ALT and AST activities,and hepatic MDA content and SOD activity were measured.Histopathological changes in the liver were also examined.Results： In liver injury mice,obvious degeneration of hepatic tissue occurred.Elevated levels of ALT and AST activities were significantly attenuated after treatment with Hyp.The activity of ALT was reduced by 89.6%,86.5% and 78.3% after treatment with Hyp 12.5,25 and 50 mg·kg-1,respectively.Hepatic histological examination showed that liver injury was serious after exposure to CCl4,and the injury was not serious after treatment with Hyp 50 mg·kg-1.Conclusion： Hyp has a protective effect on acute liver injury induced by CCl4 in mice.

关 键 词：金丝桃苷 四氯化碳 肝损伤 保护作用 

分 类 号：R282.71[医药卫生—中药学]