A miniature laser speckle fluorescence sectioning microscope for cell imaging  

作　　者：邵永红 李恒 文侨 王岩 屈军乐 牛憨笨 

机构地区：[1]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Institute of Optoelectronics, Shenzhen University

出　　处：《Chinese Optics Letters》2010年第10期944-946,共3页中国光学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 60627003);the Science and Technology Program of Guangdong Province(No. 2008A060205003) and Shenzhen (No. ZYC200903250207A)

摘　　要：We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state （DPSS） laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. Optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. High contrast fluorescence sectioning images of bovine pulmonary artery endothelial （BPAE） cells are obtained. The image quality is similar to that of the images acquired by standard laser scanning confocal microscope （LSCM）. Compared with LSCM, the laser speckle fluorescence microscope （LSFM） presented in this letter has many advantages, such as simple configurations, low cost, compact, and easy to operate, which makes it possible to have wide spread applications in biomedicine.We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state （DPSS） laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. Optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. High contrast fluorescence sectioning images of bovine pulmonary artery endothelial （BPAE） cells are obtained. The image quality is similar to that of the images acquired by standard laser scanning confocal microscope （LSCM）. Compared with LSCM, the laser speckle fluorescence microscope （LSFM） presented in this letter has many advantages, such as simple configurations, low cost, compact, and easy to operate, which makes it possible to have wide spread applications in biomedicine.

关 键 词：Endothelial cells FLUORESCENCE Image quality Light sources MICROSCOPES Pumping (laser) 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术]