A new visual investigation into nanogold-based genechip assay by atomic force and scanning tunneling microscope  

作　　者：顾大勇 谢伟东 李震 鲁卫平 周元国 季明辉 

机构地区：[1]Research Institute of Disease Control and Prevention, Shenzhen Internation Travel Healthcare Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau [2]Life Science Division, Graduate School at Shenzhen, Tsinghua University [3]Department of Chemical Defence, Navy Submarine Academy, PLA [4]Department 7, Research Institute of Surgery and Daping Hospital, The Third Military Medical University [5]Research Institute of Disease Control and Prevention, Shenzhen Entry-Exit Inspection and Quarantine Bureau

出　　处：《Chinese Optics Letters》2010年第10期964-965,共2页中国光学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 30300326 and 30972827);the Military Equipment Service Science Research and Innovation Project (No. 2-09011);the Science and Technology Project of Shenzhen (Nos. JH200504270119Aand HZ0907004),the Science and Technology Project of Nanshan, Shenzhen (No. SN200603200);the Science and Technology Project of General Administration of Quality Supervision, Inspection and Quarantine, P. R.China (No. 2008IK256-03)

摘　　要：Conformations of surface atoms in various stages of nanogold-based genechip testing are scanned by the atomic force and scanning tunneling microscope. We intuitively observe the process and differences in probe combination, nucleic acid hybridization, and silver staining, which might be useful to validate the assay method of genechip. We hope to use this technology to make the other invisible chemical or biochemical reaction become visible and convincible in the future.Conformations of surface atoms in various stages of nanogold-based genechip testing are scanned by the atomic force and scanning tunneling microscope. We intuitively observe the process and differences in probe combination, nucleic acid hybridization, and silver staining, which might be useful to validate the assay method of genechip. We hope to use this technology to make the other invisible chemical or biochemical reaction become visible and convincible in the future.

关 键 词：MICROSCOPES Nucleic acids Surface testing 

分 类 号：Q-336[生物学]