Systemic Nuclear Proteomics Researches on Change of Jurkat T Lymphocyte Cells Under Radiation  

作　　者：LIANG Feng LIU Zhong-ying LIU Ning SONG Feng-rui ZHANG Xuan LIU Zhi-qiang 

机构地区：[1]College of Pharmacy, Jilin University, Changehun 130021, P. R. China [2]Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China [3]Research Center for Genomic Medicine and MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第5期717-722,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.20675079, 20873137 and 30672600)

摘　　要：Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins, genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation.Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins, genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation.

关 键 词：Nuclear proteome Two-dimensional difference gel electrophoresis MALDI-TOF-MS Jurkat cell 

分 类 号：Q51[生物学—生物化学] S852.4[农业科学—基础兽医学]