Analysis of Vitamin K_3 by a Fluorescent Spectroelectrochemistry Method  

作　　者：ZHAO Chang-zhi LI Ying DU Hong XU Hua-jun JIAO Kui 

机构地区：[1]Key Laboratory of Eeo-Chemical Engineering, Ministry of Education, College of Chemistry ＆ Molecular Engineering, Qingdao University of Science ＆ Technology, Qingdao 266042, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第5期742-745,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Nature Science Foundation of China(No.20635020);the Open-end Fund of State Key Labo-ratory of Fine Chemicals, China(No.KF0508)

摘　　要：A simple and sensitive spectroelectrochemistry method for the determination of vitamin K3 was developed by combining electrolysis and fluoremetry. This method was based on that vitamin K3 was reduced at a glassy carbon electrode, and its product with characteristic fluorescence at 420 nm was determined with excitation wavelength at 309 nm. Under optimized electrochemical reaction conditions and fluorescent experiment parameters, the fluorescence intensity was proportional to the concentration of vitamin K3 in a range from 3.50×10^-7 to 1.05×10^-5 mol/L with a correlation coefficient of 0.9991, and detection limit was estimated to be 7.50× 10^-8 mol/L at a signal/noise ra- tio of 3. The relative standard deviation was less than 4.3%（n=5） and the recovery was in a range of 97%-105% for the determination of vitamin K3 in pharmaceutical preparations. The result is satisfactory for the determination of vitamin K3 as comparison to that from HPLC method.A simple and sensitive spectroelectrochemistry method for the determination of vitamin K3 was developed by combining electrolysis and fluoremetry. This method was based on that vitamin K3 was reduced at a glassy carbon electrode, and its product with characteristic fluorescence at 420 nm was determined with excitation wavelength at 309 nm. Under optimized electrochemical reaction conditions and fluorescent experiment parameters, the fluorescence intensity was proportional to the concentration of vitamin K3 in a range from 3.50×10^-7 to 1.05×10^-5 mol/L with a correlation coefficient of 0.9991, and detection limit was estimated to be 7.50× 10^-8 mol/L at a signal/noise ra- tio of 3. The relative standard deviation was less than 4.3%（n=5） and the recovery was in a range of 97%-105% for the determination of vitamin K3 in pharmaceutical preparations. The result is satisfactory for the determination of vitamin K3 as comparison to that from HPLC method.

关 键 词：SPECTROELECTROCHEMISTRY FLUORESCENCE Electro-reduced Vitamin K3 

分 类 号：O614.33[理学—无机化学] TQ466.6[理学—化学]