Effect of Intracoronary Infusion of Bone Marrow Mononuclear Cells or Peripheral Endothelial Progenitor Cells on Myocardial Ischemia-reperfusion Injury in Mini-swine  

作　　者：Chong-jian Li Run-lin Gao Yue-jin Yang Feng-huan Hu Wei-xian Yang Shi-jie You Lai-feng Song Ying-mao Ruan Shu-bin Qiao Ji-lin Chen Jian-jun Li 

机构地区：[1]Center of Coronary Heart Disease [2]Department of Pathology, Fuwai Hospita Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100037, China

出　　处：《Chinese Medical Sciences Journal》2010年第3期176-181,共6页中国医学科学杂志（英文版）

摘　　要：Objective To simulate and assess the clinical effect of intracoronary infusion of bone marrow mononuclear cells or peripheral endothelial progenitor cells on myocardial reperfusion injury in mini-swine model. Methods Twenty-three mini-swine with myocardial reperfusion injury were used as designed in the study protocol. About （3.54±0.90）×10^7 bone marrow mononuclear cells （MNC group, n=9） or （1.16± 1.07）× 10^7 endothelial progenitor cells （EPC group, n=7） was infused into the affected coronary segment of the swine. The other mini-swine were infused with phosphate buffered saline as control （n=7）. Echocardio- graphy and hemodynamic studies were performed before and 4 weeks after cell infusion. Myocardium infarc- tion size was calculated. Stem cell differentiation was analyzed under a transmission electromicroscope. Results Left ventricular ejection fraction dropped by 0% in EPC group, 2% in MNC group, and 10% in the control group 4 weeks after cell infusion, respectively （P〈0.05）. The systolic parameters increased in MNC and EPC groups but decreased in the control group. However, the diastolic parameters demonstrated no significant change in the three groups （P〉0.05）. EPC decreased total infarction size more than MNC did （1.60±0.26 cm2 vs. 3.71±1.38 cm2, P〈0.05）. Undermature endothelial cells and myocytes were found under transmission electromlcroscope. Conclusions Transplantation of either MNC or EPC may be beneficial to cardiac systolic function, but might not has obvious effect on diastolic function. Intracoronary infusion of EPC might be better than MNC in controlling infarction size. Both MNC and EPC may stimulate angiogenesis, inhibit flbrogenesis, and differentiate into myocardial cells.

关 键 词：bone marrow mononuclear cells endothelial progenitor cells myocardialischemia-reperfusion injury 

分 类 号：Q26[生物学—细胞生物学] S852.4[农业科学—基础兽医学]