miR26a和miR939调控H1N1型流感病毒在MDCK细胞中的复制  

miR26a and miR939 regulate the replication of H1N1 influenza virus in MDCK cell

作　　者：刘鹤[1] 宋丽萍[1] 黄文林[1,2] 

机构地区：[1]中国科学院微生物研究所病原微生物与免疫中心,北京100101 [2]中山大学附属肿瘤医院,广州510060

出　　处：《微生物学报》2010年第10期1399-1405,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30901759);中国科学院创新基金(KSCX1-YW-R-10)~~

摘　　要：【目的】研究发现microRNAs(miRNAs)可以参与调控病毒在宿主细胞内感染和复制的过程。作者研究了两条miRNAs对H1N1型流感病毒在宿主细胞内复制的影响。【方法】构建miR26a和miR939的高效表达载体,并将这两种表达载体转入MDCK细胞中,24 h后用H1N1型流感病毒感染转染后的MDCK(Madindardy canine kidney)细胞,接种72 h后,检测流感病毒的复制情况,研究miR26a和miR939对H1N1型流感病毒在MDCK细胞内复制的影响。【结果】实验结果表明,miRNAs的表达载体可以在细胞内高效表达miRNAs,不同的miRNAs对流感病毒在MDCK细胞中复制的调控作用不同,miR26a可以有效抑制流感病毒在MDCK细胞中的复制,而miR939则促进流感病毒在MDCK细胞中的复制的作用。【结论】细胞内miRNAs可以调控H1N1型流感病毒在宿主细胞中的复制过程,本文首次报导miR26a和miR939在流感病毒复制过程中的调控作用。[ Objective] MicroRNAs （miRNAs） play an important role in the process of infection and replication of virus in host cells. In this study, we cloned two miRNAs expression vectors and examined their effects on the replication of H1N1 type influenza virus in MDCK （Madin dardy canine kidney） cells. [Methods] We constructed the plasmids expressing miR26a and miR939 and performed the transfection study in MDCK cells. Subsequently, the cells were infected with H1N1 type influenza virus 24 h after transfection. Then we tested the hemagglutination titer at 72 h time point to investigate the effect of miR26a and miR939 on the replication H1N1 type influenza virus in MDCK cells. [Results] The transfection study showed that miR26a and miR939 can achieve efficiently expression in MDCK cells. miR26a and miR939 can influence the replication of H1N1 influenza virus differently in two different ways. miR26a inhibits the replication, whereas miR939 stimulates the process. [ Conclusion ] Cellular miRNAs can regulate the replication of H1N1 influenza virus in host cells, and our paper should report the role of miR26a and miR939 in this regulation for the first time.

关 键 词：MIRNA H1N1型流感病毒 MDCK细胞 病毒复制 

分 类 号：Q939.4[生物学—微生物学]