minTBP-1-PRGDN双靶向融合肽对成骨细胞增殖影响的研究  

The effect of minTBP-1-PRGDN chimeric peptide on the proliferation of osteoblastic cells

作　　者：王丹[1] 龚士强[1] 谢晖[1] 廖小福[1] 毛靖[1] 刘燕[1] 周彬[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030

出　　处：《临床口腔医学杂志》2010年第9期536-538,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金(30700957)

摘　　要：目的:探讨融合肽minTBP-1-PRGDN作为钛种植体表面涂层,对钛片表面成骨细胞增殖的影响。方法:MTT法检测MC3T3-E1成骨细胞在不同涂层的钛片表面粘附24h、48h、72h后增殖数量的差异;金相显微镜观察各组细胞的生长形态及密度;Real-timePCR定量比较细胞增殖相关基因cyclin D1的表达差异。结果:各组中成骨细胞均随着时间延长而出现增殖,24h时minTBP-PRGDN组细胞增殖数量明显高于空白对照组(P<0.05);72h时,minTBP-PRGDN组的cyclin D1表达水平最高。结论:融合肽minTBP-PRGDN能有效促进钛表面成骨细胞的增殖。Objective：To explore the effect of minTBP-1-PRGDN on the proliferation of osteoblastic cells.Method：minTBP-PRGDN,minTBP and PRGDN were used as coating material on titanium disks,and the disks uncoated were used as control.MC3T3-E1 osteoblasts were cultured on those disks for 24 h,48 h and 72 h.Cell numbers were evaluated by MTT assay,and the cell morphology and density were observed with metallurgical microscope.The expression of cyclin D1 gene was detected by real-time PCR.Result：Cell numbers in all groups increased with the the passing of time.At 24 h,cell number in minTBP-1-PRGDN group was maximum so that significant difference was found when comparing with the control group（P 0.05）.At 72 h,the gene expression of cyclin D1 was highest in all groups.Conclusion：Chimeric peptide minTBP-1-PRGDN could promote the proliferation of osteoblastic cells on Ti.

关 键 词：融合肽 成骨细胞 细胞增殖 钛 

分 类 号：R318.04[医药卫生—生物医学工程] R780.2[医药卫生—基础医学]