SOX11结合p53并上调其转录活性  

SOX11 Interacts with p53 and Increases its Transcriptional Activity

作　　者：常艳[1] 张维娜[1] 李腾[1] 高彦飞[1] 李慧艳[1] 满江红[1] 梁冰[1] 巩伟丽[1] 于鸣[2] 潘欣[1] 

机构地区：[1]国家生物医学分析中心,北京100850 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《生物技术通讯》2010年第5期601-604,共4页Letters in Biotechnology

基　　金：国家自然科学基金(30871275)

摘　　要：目的:研究SOX11对p53转录活性的影响,并检测二者的体外相互作用。方法:在H1299(p53缺失)和H460(含野生型p53)2种细胞中分别过表达SOX11和p53,用双萤光素酶方法测定p53的转录活性;用大肠杆菌DH5α表达GST和GST-p53融合蛋白并将其纯化,用GST pull-down实验检测SOX11与p53在体外是否存在相互作用。结果:萤光素酶实验结果表明,在H1299和H460细胞中,过表达SOX11分别能促进外源p53和内源p53的转录活性;GST pull-down实验表明SOX11能在体外与p53发生相互作用。结论:SOX11能在体外与p53发生相互作用并促进p53的转录活性,为进一步研究p53的功能提供了新的线索。Objective： To explore whether SOX11 regulates p53 activity and examine their interaction in vitro.Methods： H1299（p53 null） and H460（p53 wild type） cells were transfected with Myc-SOX11 and p53 response reporter,and the p53 transcriptional activity was measured by luciferase reporter assay.We purified GST and GST-p53 fusion protein expressed in E.coli DH5α,and then performed GST pull-down assay to assess the interaction between SOX11 and p53 in vitro.Results： The results of luciferase reporter assay for H1299 and H460 cells showed that SOX11 overexpression could increase exogenous and endogenic p53-mediated transcription respectively.The result of GST pull-down assay indicated that SOX11 could especially bind to GST-p53 fusion protein but not GST protein.Conclusion： These findings reveal that SOX11 can interact with p53 in vitro and increase its transcriptional activity,which may provide a new insight on the regulation of p53.

关 键 词：SOX11 P53 相互作用 转录活性 

分 类 号：Q75[生物学—分子生物学]