一种新的Srrm1/SRm160可变剪接体经历无义突变介导的mRNA降解  

A Novel Splicing Variant of Srrm1/ SRm160 Targets to Nonsense-Mediated mRNA Decay

作　　者：何章荣[1] 瞿秀华[1] 刘萱[1] 李晓明[1] 靳彦文[1] 李平[1] 曹诚[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2010年第5期605-609,共5页Letters in Biotechnology

摘　　要：目的:研究基因Srrm1/SRm160的可变剪接。方法:应用RT-PCR研究Srrm1/SRm160的可变剪接,通过蛋白质的翻译抑制和RNA干扰研究剪接异构体是否经历无义突变介导的mRNA降解(NMD)过程。结果:获得Srrm1/SRm160新的可变剪接异构体,该异构体产生提前终止密码子,翻译抑制和RNA干扰证实含有提前终止密码子的剪接体经过NMD而降解。结论:Srrm1/SRm160通过可变剪接和NMD调节自身的表达水平,作为剪接因子进一步调节其他基因的可变剪接。Objective： To study the alternative splicing of gene Srrm1 / SRm160.Methods： RT-PCR was used to study the alternative splicing of Srrm1 / SRm160,followed by protein translation inhibition and RNA interference（RNAi） to determine if the variant is an nonsense-mediated mRNA decay（NMD） target.Results： Srrm1 / SRm160 generated a novel variant through alternative splicing.The variants contained a premature termination codon and triggerd the NMD which was confirmed by the protein translation inhibition and RNAi.Conclusion： Srrm1 / SRm160 regulates its own expression by alternative splicing and NMD,and then regulates other genes＇ alternative splicing as a splicing factor.

关 键 词：可变剪接 提前终止密码子 无义突变介导的mRNA降解 

分 类 号：Q75[生物学—分子生物学]