ELISPOT检测高致病性猪繁殖与呼吸障碍综合征病毒感染后猪γ-干扰素的分泌情况  

Detection of Porcine IFN -γ in Pigs Infected by High Pathogenic Porcine Reproductive and Respiratory Syndrome Virus with the Application of ELISPOT

作　　者：郑霖[1] 马玉媛[1] 颜奇坡[1] 叶小丽[1] 郭逸[1] 田克恭[2] 章金刚[1] 

机构地区：[1]军事医学科学院野战输血研究所国家生物医学分析中心病毒安全检测实验室,北京100850 [2]中国动物疫病预防控制中心,北京100094

出　　处：《生物技术通讯》2010年第5期711-714,共4页Letters in Biotechnology

基　　金：国家科技支撑计划(2007BAD86B01-3)

摘　　要：目的:研究高致病性猪繁殖与呼吸障碍综合征病毒(PRRSV)感染后猪外周血单个核细胞(PBMC)特异性分泌γ-干扰素(IFN-γ)的免疫反应。方法:将仔猪接种PRRSV,于病毒接种前后各时间点分别采血并分离PBMC,采用酶联免疫斑点法(ELISPOT)检测PBMC分泌IFN-γ的情况。结果:猪感染高致病性PRRSV后,PBMC分泌IFN-γ的能力明显增强,对照组无明显变化。结论:该结果可为研究高致病性PRRSV致病机理提供参考,为评价PRRSV疫苗诱发的细胞免疫效应提供依据。Objective： To investigate the production of IFN-γ from peripheral blood mononucler cells（PBMC） in pigs infected by high pathogenic porcine reproductive and respiratory syndrome virus（PRRSV）.Methods： Pigs were inoculated with high pathogenic PRRSV.PBMC were isolated from blood samples collected before or post inoculation.Porcine IFN-γ screened by PBMC was detected by enzyme-linked immunospot assay（ELISPOT）.Results： The frequency of PRRSV-specific IFN-γ sceening cells increased when pigs were infected by high pathogenic PRRSV.The control group didn＇t show significant changes.Conclusion： The results were helpful for exploring the pathogenic mechanism of high pathogenic PRRSV and for evaluating the cell-mediated immune response induced by PRRSV vaccine.

关 键 词：酶联免疫斑点法 猪繁殖与呼吸障碍综合征病毒 Γ-干扰素 

分 类 号：R392.1[医药卫生—免疫学]