Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding  

作　　者：Jun-mei WANG Jian-ming YANG Jing-huan ZHU Qiao-jun JIA Yue-zhi TAO 

机构地区：[1]Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2010年第10期792-800,共9页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (Nos. 30700485 and 30771333);the Zhejiang Provincial Natural Science Foundation (No. Y306641),China

摘　　要：The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat （SSR） genotyping data. The amplified fragments from SSR primers were highly polymorphic in the badey accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 badey accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat(SSR) genotyping data.The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated.A total of 85 alleles were detected at 35 SSR loci,and allelic variations existed at 29 SSR loci.The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions.A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups.Seven malting barley varieties from China fell into the same subgroup.It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources,suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.

关 键 词：Barley （Hordeum vu/gare L.） Genetic similarity Simple sequence repeat （SSR） marker Cluster analysis Genetic diversity 

分 类 号：Q37[生物学—遗传学]