应用超声波技术强化提取余甘多糖  

Ultrasound-assisted Extract of Water-soluble Polysaccharides from Phyllanthus emblica

作　　者：李永裕[1,2] 陈建烟[1,2] 温凤英[1,2] 关夏玉[1] 王曾金[1] 吴少华[1,2] 

机构地区：[1]福建农林大学园艺学院,福州350002 [2]福建农林大学园艺植物天然产物研究所,福州350002

出　　处：《热带亚热带植物学报》2010年第5期552-558,共7页Journal of Tropical and Subtropical Botany

基　　金：国家科技支撑计划项目(2007BAD07B01);福建省自然科学基金项目(2009J01065);国家大学生创新性实验计划项目(081038902)资助

摘　　要：通过单因素和正交实验研究了超声波时间、超声波功率、超声波提取温度和料液比对余甘(Phyllanthus emblica L.)多糖收率的影响。结果表明,多糖收率的主要影响因素为超声波功率、超声波时间、料液比、超声波提取温度。余甘多糖的最佳提取工艺条件为:超声波功率475W、超声波时间70min、料液比1:20、超声波提取温度80℃,余甘多糖收率为95.86mgg-1DW。紫外和红外光谱分析表明,余甘多糖是含有糖醛酸的α型吡喃酸性杂多糖,超声波处理没有改变多糖结构。Emblica polysaccharides （EPS） have biological activities on free radical scavenging, anti-oxidation and anti-tumor. The effect factors on yield of emblica polysaccharides from Phyllanthus emblica extracted by ultrasound were studied. The results showed that the factors included ultrasonic power, ultrasonic time, solid-liquid ratio, ultrasonic extraction temperature. The optimal extraction condition were ultrasonic power of 475 W with solid （g） to liquid （mL） ratio of 1： 20 at 80℃ for 70 min, with the yield of 95.86 mg g^-1 DW. Ultraviolet spectrum and FT-IR （fourier transform infrared spectroscopy） analysis showed that EPS was a α type acidic heteropolysaccharide containing pyran and glycuronic acid. The structural modification of EPS by ultrasonic radiation was not observed.

关 键 词：余甘 多糖 超声波 提取 红外光谱 

分 类 号：S567.19[农业科学—中草药栽培]