北京地区2009年5-12月急性上呼吸道感染病毒病原学调查  

Study on the viral etiology of the acute upper respiratory infections in Beijing area during May to December 2009

作　　者：吕燕宁[1] 黄芳[1] 高志勇[1] 张新[1] 崔淑娟[1] 郭靖[1] 张代涛[1] 李伟红[1] 曲梅[1] 严寒秋[1] 卢桂兰[1] 王全意[1] 

机构地区：[1]北京市疾病预防控制中心,北京100013

出　　处：《中国卫生检验杂志》2010年第10期2476-2478,2481,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解2009年5月-12月间北京地区人群急性上呼吸道感染非甲型流感病毒感染的病毒病原学情况,探讨常见急性上呼吸道感染病毒性病原谱的构成,为北京地区急性呼吸道传染病预防策略的制定提供依据。方法:采用多重RT-PCR方法,对626份甲型流感病毒检测阴性的咽拭子样本同时检测人偏肺病毒、腺病毒、人冠状病毒229E/NL63、副流感1、2、3亚型,乙型流感、人冠状病毒OC43/HUK1,鼻病毒A/B、呼吸道合胞病毒A、B亚型等11种常见呼吸道病毒。结果:626份甲型流感阴性样本中检出其他病毒的阳性样本有115份,阳性率为18.37%,其中有2种以上病毒混合感染14份,占阳性样本的12.17%。5、6、7月份以副流感病毒检出率最高,其中15岁以下患者以1型副流感病毒检出阳性率最高,15岁以上患者中以3型副流感病毒检出阳性率最高;8、9、12月份以鼻病毒检出率为最高。结论:副流感病毒与鼻病毒为2009年5-12月间北京地区人群急性上呼吸道感染的非甲型流感的主要病毒性致病原。Objective：To study the situation of the viral etiology of the acute upper respiratory infections in Beijing area during May to December 2009,and to discuss the respiratory viral epidemiology in Beijing to provide the background data for the preventive strategy.Methods：11 kinds of common respiratory viruses： human metapneumovirus,adenovirus,parainfluenza virus l,2,3,influenza virus B,respiratory syncytial virus A,B,human rhinovirus A/B,human coronavirus OC43/HUK1,229E/NL63 were detected in 626 influenza A negative throat swabs simultaneously by multiplex RT-PCR.Results：115 positive samples were identified from the 626 swabs,the positive rate was 18.37%.The coinfections caused by more than 2 kinds of viruses were 14,which occupied 12.17% of the positive samples.PIV was the mostly detected pathogen in May,June and July,HRV was the mostly detected pathogen in August,September and December.PIV1 was the mostly detected pathogen in patients under 15,PIV3 was the mostly detected pathogen in patients older than 15.Conclusion：PIV and HRV were the main pathogens leading to the acute upper respiratory infections in Beijing area during May to December 2009 besides influenza A virus.

关 键 词：甲型H1N1流感病毒 急性上呼吸道感染 多重PCR法 病毒病原学 

分 类 号：R373.1[医药卫生—病原生物学]