多色荧光PCR检测HBV耐药变异的临床研究  

Clinical study of multicolor fluorescence PCR on rapid detection of drug-resistant mutations in hepatitis B virus

作　　者：张腊红[1] 陈兆军[1] 朱明利[2] 刘霞[1] 潘峰[1] 徐立群[1] 赵晓飞[1] 林冰[1] 

机构地区：[1]杭州师范大学附属医院,杭州310015 [2]浙江省杭州市第六人民医院,杭州310014

出　　处：《中国卫生检验杂志》2010年第10期2494-2495,共2页Chinese Journal of Health Laboratory Technology

基　　金：杭州市医学重点专科专病专项(20070433Q12)

摘　　要：目的:多色荧光PCR技术分析拉米夫定,阿德夫韦和恩替卡韦治疗不同时期HBV DNA耐药变异情况。方法:将所有病例按照服用拉米夫定,阿德夫韦和恩替卡韦进行分组,定期抽取血清,采用多色荧光PCR技术对其HBV进行检测。结果:拉米夫定治疗6个月后,1例发生耐药变异;12个月后耐药率达到18.8%,24个月后高达40.8%;阿德夫韦服用两年后出现少量耐药,三年后耐药率达到9.7%;恩替卡韦服用期间耐药突变很少。结论:拉米夫定,阿德夫韦和恩替卡韦服用一定的时间均会不同程度的出现耐药变异,定期对服药病人进行耐药检测,有利于及时调整治疗方案,增加疗效,减少不必要的浪费,减轻病人负担。Objective：Detect mutations associated with lamivudine,adefovir and entecavir by Multicolor fluorescence PCR.Methods： All cases were divided into three groups according to different drug exposure,HBV mutations were detected by Multicolor fluorescence PCR termly.Results： One case had mutation after treating by lamivudine for 6 months;resistance rate was 18.8% after 12 months,which reached 40.8% after 24 months.There wre a little mutations after treating by adefovir,the resistance rate was 9.7% after three years.Mutations associated with entecavir were rarely detected.Conclusion： Cases treated with lamivudine,adefovir and entecavir will have mutation in different degree after a period.Detecting drug-resistant mutations of patients regularly were important.It was helpful to change the therapeutic scheme in time,increasing curative effect,reducing unnecessary waste.

关 键 词：多色荧光PCR 耐药 HBV 拉米夫定 阿德夫韦 恩替卡韦 

分 类 号：R512.62[医药卫生—内科学]