肾安提取液对糖尿病肾病模型小鼠肾脏AGEs、RAGEs的影响  

Effect of Shen'an Extract on Expression of Renal AGE and RAGEs in Nephropathy Mice

作　　者：王小琴[1] 唐庆[2] 马俊[2] 向少伟[2] 

机构地区：[1]湖北省中医院,湖北武汉430061 [2]湖北中医药大学,湖北武汉430065

出　　处：《湖北中医学院学报》2010年第5期3-6,共4页Journal of Hubei College of Traditional Chinese Medicine

基　　金：湖北省自然科学基金(2008CHB414)

摘　　要：目的观察肾安提取液对糖尿病肾病(DN)模型小鼠肾脏AGEs、RAGEs的影响。方法小鼠随机分为正常对照组、DN模型组、厄贝沙坦组、肾安高剂量组、肾安中剂量组、肾安低剂量组,采用腹腔注射链脲佐菌素(STZ)建立小鼠DN模型,药物干预4周后,Realtime-PCR检测肾脏RAGEs mRNA表达,免疫组化法检测肾脏RAGEs的表达,分光光度法检测肾组织AGEs含量。结果 DN组小鼠肾脏AGEs、RAGEs mRNA、RAGEs表达较正常对照组显著升高(P<0.01),肾安提取液各剂量组肾脏AGEs、RAGEs mRNA、RAGEs表达较DN组显著降低(P<0.05,P<0.01)。结论肾安提取液能保护DN小鼠肾脏,其机制可能与降低肾皮质RAGEs mRNA、RAGEs表达,降低肾组织AGEs含量有关。Objective To investigate the influences of Shen＇an extract on renal AGEs and RAGEs in diabetic nephropathy（DN） mice.Method The mice were induced diabetic nephropathy by intraperitoneal injection of streptozotocin（STZ） and were randomized into 6 groups： normal control group;DN model group;Irbesartan group;high-dose Shen＇an group;medium-dose Shen＇an group;low-dose Shenan group.After 4 weeks intervention with medications,the expression of renal RAGEs mRNA was detected by Realtime-PCR,renal RAGE by immunohistochemistry,and renal content of AGEs by spectrophotometry.Result Expression of renal RAGEs mRNA and RAGEs of diabetic nephropathy mice increased obviously in comparison with that of normal control group（P0.01）.The three doses Shen＇an groups showed significant decrease in renal RAGEs mRNA and RAGEs expression as compared with that in DN group（P0.05,P0.01）.Conclusion Shen＇an extract could protect kidney of diabetic nephropathy mice.The underlying mechanism may be related to its loweirng renal expression of RAGEs mRNA,RAGEs and AGEs content.

关 键 词：肾安提取液 糖尿病肾病 糖基化终产物 糖基化终产物受体 

分 类 号：R285.5[医药卫生—中药学]