MTBDR plus方法快速检测北京地区临床结核分枝杆菌分离株利福平和异烟肼耐药性效果评价  

Evaluation on the MTBDRplus assay for rapid detection of resistance to rifampin and isoniazid in Mycobacterium tuberculosis Clinical isolates in Beijing

作　　者：菅记涌[1] 王嫩寒[1] 易俊莉[1] 邢青[1] 刘晓雪[1] 王甦民[1] 洪峰[1] 

机构地区：[1]北京结核病控制研究所,北京100035

出　　处：《中国防痨杂志》2010年第10期611-614,共4页Chinese Journal of Antituberculosis

摘　　要：目的评估MTBDR plus试剂盒在北京地区快速检测利福平和异烟肼的耐药性的效果。方法筛选169例临床结核分枝杆菌分离株,以国际标准的比例法作为对照,探讨该试剂盒在临床上应用的敏感性和特异性以及突变分布频率。结果在北京地区使用MTBDR plus检测利福平和异烟肼的敏感性和特异性分别为96.9%、85.3%和93.2%、98.1%。rpoB基因频率最高的突变位点是S531L(55.2%),其次是D516V(8.3%)、H526Y(7.3%)和H526D(3.1%)。katG基因频率最高的突变位点是S315T1(62.9%),而inhA是C15T位点(21.6%)。结论 MTBDR plus是一个敏感、特异和快速的诊断利福平、异烟肼耐药性和MDR的有效方法。Objective To evaluate the effect of MTBDRplus assay in rapid detection of resistance to rifampin （RIF） and isoniazid （INH） in Mycobacterium tuberculosis clinical isolates collected in Beijing. Methods A total of 169 clinical isolates were enrolled in the study. Using the tranditional drug susceptibility testing as the control, the sensitivity and specificity of the MTBDRplus assay and the variety of gene mutations were assessed. Results The sensitivity and specificity of MTBDRplus assay for the detection of RIF and INH resistance were 96.9%, 85.3% and 93.2%, 98. 1%, respectively. The S531L mutation of rpoB was the most frequent （55.2%）, followed by mutations D516V （8.3%）, H526Y （7.3%） and H526D （3.1%）. The frequent mutations of katG gene and inhA promoter region were S315T1 （62.9%） and C15T （21.6%） in INH-resistant isolates, respectively. Conclusion The MTBDRplus assay is a sensitive, specific and rapid tool for the diagnosis of RIF, INH resistance and multi-drug resistance in clinical isolates.

关 键 词：分枝杆菌 结核 抗药性 细菌 微生物敏感性试验 利福平 异烟肼 

分 类 号：R446.5[医药卫生—诊断学]