荧光定量PCR技术在肺结核诊断中的临床应用研究  

The clinical application of fluorescent quantitative PCR in diagnosis of pulmonary tuberculosis

作　　者：吴驰[1] 张红梅[1] 詹能勇[1] 邓永聪[1] 单万水[1] 余卫业[1] 

机构地区：[1]深圳市第三人民医院,深圳518020

出　　处：《中国防痨杂志》2010年第10期647-650,共4页Chinese Journal of Antituberculosis

基　　金：深圳市科技计划重点项目资助(200701013)

摘　　要：目的评价荧光定量PCR技术在肺结核病诊断中的应用价值。方法采用荧光定量PCR、金胺0染色荧光镜检和培养法同时检测860例肺结核患者的痰标本，对检测结果进行比较。结果荧光定量PCR检测灵敏度可达10～100cfu／ml，重复性好，对其他呼吸道病原体检测结果均为阴性。肺结核患者痰标本荧光定量PCR检测阳性率要高于金胺O染色荧光镜检和培养法的阳性率，差异有统计学意义（P〈0．01）。结论应用荧光定量PCR方法检测结核杆菌，具有特异、灵敏、简便、快速的特点，可作为结核病诊断的方法之一。Objective To study clinical value of fluorescent quantitative PCR （FQ-PCR） on detection of Mycobacterium tuberculosis complex （MTB）. Methods 860 sputum samples from the patients with pulmonary tuberculosis were detected by FQ-PCR, staining and culture, respectively. The sensitivity and specificity of FQ-PCR were assessed by comparing the results. Results The sensitivity of FQ-PCR reached 10- 100 CFU/ml. The results of other pathogens in respiratory tract were negative by FQ-PCR. The positive rate of FQ-PCR in testing sputum samples was higher than that of staining and culture, and there was a significant difference （P〈0.01）. Conclusion FQ- PCR, characterized by distinct specificity, high sensitivity, simplicity and quick-response, can be used as a conventional method for detecting MTB.

关 键 词：结核 肺/诊断 分枝杆菌 结核 聚合酶链反应 

分 类 号：R440[医药卫生—诊断学]