降糖明目汤对C57BL/6糖尿病小鼠视网膜抗氧化能力影响的实验研究  

Effect of jiangtangmingmutang on the antioxidation in retina of C57BL/6 diabetic mice

作　　者：侯艳丽[1] 罗丽华[1] 王艳玲[1] 

机构地区：[1]首都医科大学附属北京友谊医院眼科,北京100050

出　　处：《临床眼科杂志》2010年第5期464-466,共3页Journal of Clinical Ophthalmology

摘　　要：目的 (1)探讨降糖明目汤对糖尿病视网膜组织抗氧化能力的影响。(2)研究糖尿病小鼠胰腺及肾脏抗氧化能力。方法 30只C57BL/6小鼠分为对照组、糖尿病组及中药治疗组。糖尿病组、中药治疗组通过腹腔注射链脲佐菌素制作糖尿病模型,中药治疗组每日灌胃降糖明目汤1次。观察小鼠血糖变化,于第80天测定视网膜组织、胰腺及肾脏超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果糖尿病组,中药治疗组空腹血糖始终>16.7 mmol/L,中药治疗组血糖较糖尿病组稍低,但差异无统计学意义(P>0.05),第80天中药治疗组视网膜及胰腺组织SOD活性高于糖尿病组(P<0.05),MDA含量低于糖尿病组(P<0.05)。肾组织SOD活力及MDA含量无明显变化(P>0.05)。结论 (1)降糖明目汤无降血糖作用,但能够提高糖尿病小鼠视网膜组织SOD活性,减少MDA的产生,保护糖尿病视网膜的自由基损伤。(2)肾在氧化损伤过程中具有一定代偿能力。Objective（1）To investigate the effect of Jiangtangmingmutang on the antioxidation in retina of diabetic mice.（2）To investigate the antioxidation of pancreas and kidney.Methods 30 C57BL/6 mice were divided into three groups： normal control group,diabetic mice group and treatment group.Diabetic mice were induced by streptozocin（STZ）.Treatment group treated with Jiangtangmingmutang by intra gastric feeding daily.Fasting blood glucose level（FBG） were observed periodically,The SOD activity and MDA content in retina pancreas and kidney were examined after 80 days.Results FBG in the diabetic mice group and treatment group were kept over 16.7 mmol/L over 80days.FBG in treatment group was lower then diabetic mice group,but have no statistical sense（P0.05）.The SOD content of retina and pancreas were higher than the diabetic group（P0.05）and the MDA content were lower than the diabetic group（P0.05）.The SOD and MDA content in kidney have no change.Conclusion（1）Jiangtangmingmutang cannot decrease the FBG,but increase the SOD activity of the diabetic mice＇ retina,thus decrease the production of the MDA and reduce the free radical damage of retina tissue.（2）Kidney can resist free radical damage.

关 键 词：降糖明目汤 糖尿病 视网膜 超氧化物歧化酶 丙二醛 实验研究 

分 类 号：R285.5[医药卫生—中药学]