重复序列引物聚合酶链反应在检测肺炎克雷伯菌院内流行中的应用  

Preliminary Application of rep-PCR in Epidemiological Research of Klebsiella Pneumoniae

作　　者：王晶[1] 韩丽霞[1] 张凤华[1] 李健[1] 

机构地区：[1]大连医科大学第一临床学院检验科,辽宁大连116011

出　　处：《现代检验医学杂志》2010年第5期28-30,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 用重复序列引物聚合酶链反应（rep-PCR）方法分析从大连医科大学第一临床学院不同患者分离出来的肺炎克雷伯菌是否起源于同一菌株.方法 用微生物分析系统检测其对抗生素的敏感程度,选用肠杆菌科广泛存在的基因间重复片段为引物进行扩增,对肺炎克雷伯菌进行基因分型.结果 建立了rep-PCR法方法,显示可将肺炎克雷伯菌扩增出丰富的区带.分离到的33株产ESBL肺炎克雷伯菌分属6个基因型.结论 rep-PCR是一种快速、简便、可靠的基因分型方法,是分子水平追踪医院感染较理想的方法.Objective To investigate if the Klebsiella pneumoniae isolated from different patients in Dalian Medical University First Hospital have the same origin byusing rep-PCR. Methods Microbial analysis system detected the sensitivity to antibiotics. By applying the widespread enterobacter repetitive intergenic consensus as a primer were for Klebsiella pneumoniae genotype. Results Established a rep-PCR method,the analysis of the PCR productions indicated that it would create useful DNA ban-prints and all those 33 Klebsiella pneumoniae were of six origins. Conclusion Rep-PCR is a fast,simple and reliable genotyping and approach is to track the molecular level,an ideal tool for nosocomial infection.

关 键 词：重复序列引物聚合酶链反应 肺炎克雷伯杆菌 医院感染 

分 类 号：R503[医药卫生—内科学] R378.996[医药卫生—临床医学]