聊城地区淋球菌流行株耐药性研究及质粒谱型分析  

Research on the Antibiotic Resistance and Plasmid Profiles of Prevail Neiossria Gonorrhoeae in Liaocheng

作　　者：初瑞雪[1] 孟卫东[1] 

机构地区：[1]聊城市人民医院,山东聊城252000

出　　处：《现代检验医学杂志》2010年第5期90-92,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 了解聊城地区淋球菌流行株对常用抗生素的耐药性,分析当地淋球菌耐药质粒谱特点.方法 采用琼脂稀释法检测淋球菌对四环素、环丙沙星、头孢曲松、大观霉素的最小抑菌浓度（MIC）;纸片碘量法测定菌株的β-内酰胺酶;用小量碱裂解法,对淋球菌进行质粒抽提,对提取质粒进行琼脂糖凝胶电泳分析;探讨耐药谱与质粒谱之间的关系及与其他地区间的差异.结果 52株淋球菌检测出产青霉素酶淋球菌（PPNG）菌株17株（32.69%）,高度耐四环素（TRNG）菌株15株（28.85%）,环丙沙星耐药51株（98.08%）,大观霉素耐药1株（1.92%）、头孢曲松未发现耐药菌株;质粒检出率86.54%（45/52）,共检测到4种分子量质粒,6种质粒谱型,以2.6＋4.5,2.6＋4.5＋25.2和4.5＋25.2＋24.5为主,分别占30.77%,25.00%和15.38%.结论 聊城地区淋球菌耐药情况与国内其他地区比较基本相类似,大观霉素和头孢曲松仍为临床首选;聊城地区淋球菌耐药与多数地区耐药质粒谱有差异,聊城地区淋球菌流行株耐药主要以高水平的质粒耐药为主.Objective To detect the resistance of Neisseria gonorrhoeae （NG） and to analyse the characteristics of local gonococcal resistance plasmids. Methods Detected minimum inhibitory concentration （MIC） of NG to tetracycline, eiprofloxacin,ceftriaxone and spectinomycin by agar dilution method and detected lactamase by paper iodometry. Then extracted plasmids from NG with alkaline lysis method,and analysed the plasmid with agarose gel electrophoresis. Explored the differences between other regions last. Results MIC of 52 NG,PPNG strains 17（32.69%）,TRNG 15 strains （28. 85%）, Ciprofloxacin resistance 51 （98.08 % ）, Spectinomycin resistance 1 （1.92 % ）, Ceftriaxone resistant strains was not found. Detection rate of plasmid was 86.54% and 4 kinds of molecular weight were detected among plasmids ,6 kinds of plasmid profiles,2.6±4. 5,2.6±4. 5±25.2,4.5±25.2±24.5 accounted for 30. 77% ,25% and 15.38% respectivly. Conclusion Resistance of NG in Liaocheng was similar to other area,spectinomycin and ceftriaxone were the first choice of clinical. The resistant strains of NG in Liaocheng were high levels of plasmid-based.

关 键 词：奈瑟氏淋球菌 耐药性 最小抑菌浓度 质粒图谱 

分 类 号：R378.16[医药卫生—病原生物学] R446.5[医药卫生—基础医学]