二维及三维超声对组织工程化神经导管修复外周神经缺损的评价  

Evaluation of Tissue Engineering Nerve Conduit in Repairing Peripheral Neurologic Defect with 2-D and 3-D Ultrasound

作　　者：曹敏丽[1] 赵红斌[1] 王红义[1] 刘旭东[1] 马敬[1] 白孟海[1] 

机构地区：[1]兰州军区总医院超声诊断科,兰州市730050

出　　处：《中国超声医学杂志》2010年第10期879-882,共4页Chinese Journal of Ultrasound in Medicine

基　　金：兰州军区医药卫生科研项目(No.LXH-2007007)

摘　　要：目的探讨二维及三维彩色多普勒超声评价组织工程化神经导管促进缺损神经再生的可行性。方法利用壳聚糖包被中药单体川芎嗪制备缓释微球与胶原蛋白混合构建组织诱导性神经导管;大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)体外诱导48 h后,MSCs与细胞外基质(extracellular matrix,ECM)胶100:1混合均匀接种于导管内腔,3d后利用显微缝合方法将神经导管与8只大鼠缺损坐骨神经连接,其中4只为无MSCs的神经导管作为对照。分别于连接后7、30和90d时,应用二维及三维超声观察缺损神经再生状况。结果超声结果显示,7d后可见强回声的神经导管,导管两端有强回声神经纤维嵌入;30d后神经导管呈较强回声界限尚清但均匀,同时可见导管两端有强回声。无MSCs组和MSCs组导管两端强回声长度分别为(2.4±0.2)mm、(2.1±0.2)mm和(2.7±0.3)mm、(2.9±0.1)mm,两组差异具有统计学意义(P<0.01);90d后神经管模糊、断续、欠不规则,回声较强,提示导管材料发生降解。无MSCs组神经管内可见2～3个强回声,两端回声长度分别为(3.5±0.2)mm和(3.6±0.1)mm;MSCs组神经导管内可见数条强回声,两端强回声长度分别为(4.0±0.3)mm和(3.8±0.2)mm,两组具有统计学意义(P<0.05)。结论超声在评价组织工程化神经导管对缺损神经的修复方面具有潜在应用价值。Objective To study the possibility of ultrasound in evravuating tissue engineering nerve conduit to promote the regeneration of defect nerve. Methods With application of the collagen protein and chitosan microsphere coated with ligustrazine to fabricate the tissues induction nerve conduit,mesenchymal stem cells （MSCs） were inducted for 48 hours in vitro,MSCs were combined with extracellular matrix glue （ECM） （100 ： 1） to implant in the nerve condiut,After 3 days the defect sciatic nerves were linked with the nerve condiut in 8 rats,among which 4 rats as control were without MSCs in nerve condiut, two dimensional and three dimensional ultrasound were used to observe the regeneration state of the defect nerve in 7,30 and 90 days respectively after connection. Results The result of the ultrasound showed that the hyperechoic nerve condiut was clear and the nerve fibers inserted in amphi conduit after 7 days. 30 days later the margin of the nerve conduit was clear and asymmetry,meanwhile the hyperechoic line entered in conduit. The length of the hyperechoic were 2.4±0.2mm、2.1±0.2mm and 2.7±0.3mm、2.9±0.1mm respectively in the without MSCs and MSCs groups, which differed signaficantly in two groups（P〈0.01）. After 90 days the nerve conduit was ambiguity, interrupt and irregular, became relatively stronger, which suggested that it was degraded. 2 to 3 dense echoes were found in the nerve conduit in the without MSCs group. The length of the hyperechoic was 3.5±0.2 mm and 3.6±0. 1 mm respectively. There were several the dense echoes in MSCs group. The length was 4.0±0.3 mm and 3.8±0.2 mm respectively, which was different to compare with the without MSCs group（P〈 0.05）. Conclusions Utrasound plays an important role in evaluating the tissue engineering nerve conduit to repair the defect nerve.

关 键 词：彩色多普勒超声 组织工程化神经导管 神经缺损修复 

分 类 号：R651.3[医药卫生—外科学]