一种新的测定刀豆氨酸含量的方法  

A new Method of L-canavanine Determination

作　　者：陈东芳[1] 文汉[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《中国农学通报》2010年第19期99-102,共4页Chinese Agricultural Science Bulletin

摘　　要：主要研究了2,4-二硝基氟苯与刀豆氨酸在pH9.5硼砂缓冲溶液中反应的最佳条件,从而拟建立一种新的、快速简便的测定刀豆氨酸的方法。主要运用了分光光度法测定络合物在571nm处的吸光度。结果表明：在50℃水浴30min后,刀豆氨酸与2,4-二硝基氟苯发生显色反应,即形成绿色1：1的稳定络合物。其最大吸收峰在571nm,表观摩尔吸光系数∈=1.086×104L/（mol·cm）,在13.75~220μg/mL范围内遵守比尔定律,对应线性回归方程为：y=0.0051x＋0.0682;相关系数为：R2=0.9939。加标回收率为96.6%~101.56%范围之内,相对标准偏差在1.768%之内,从而建立了刀豆氨酸简单、快速测定的新方法。This paper studied optimum conditions about 2,4 - dinitrofluorobenzene and L-canavanine reaction in pH9.5 borax buffer solution, for establishment of a new, fast and easy determination of L-canavanine method. The method used spectrophotometry determination 571 nm absorbance. The results showed that L-canavanine with 2,4 - dinitrofluorobenzene occurred color reaction at 50℃ for 30 min, forming a stable green complex with 1：1. The maximum absorption peak at 571 nm and the apparent molar absorption coefficient ∈ =1.086×10 4 L/（mol·cm）. It obeys beer ＇ s law in 13.75-220 μ g/mL, the corresponding linear regression equation, y=0.0051x＋0.0682; correlation coefficient R 2 =0.9939. Recoveries were 96.6%-101.56%, relative standard deviation of 1.245%-1.768% range. Thus a simple new method for rapid determination L-canavanine was established.

关 键 词：刀豆氨酸 分光光度法 2 4-二硝基氟苯 

分 类 号：Q946.889[生物学—植物学]