柑橘绿霉病菌拮抗放线菌MY-5的筛选与鉴定  

Screening and Identification of Actinomyces MY-5 Antagonistic Against Penicillium digitatum Sacc

作　　者：李辉[1] 耿鹏[1] 郝卫宁[1] 胡美英[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广州510642

出　　处：《中国农学通报》2010年第19期280-284,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目(30871660);广东省科技计划项目(2008B020900007)

摘　　要：筛选拮抗活性菌株和寻找新的活性代谢产物,可为果蔬采后病害防治提供新的资源。此文主要研究广东省各地区水果园和蔬菜地采集土壤23份并分离获得放线菌212株。以柑橘绿霉病菌为目标菌,采用抑菌带测定法,筛选获得9株具有拮抗活性的放线菌菌株,其中菌株MY-5拮抗活性最强,抑菌带宽达18.67mm,同时对其它13种果蔬采后病害也有较强的拮抗作用。通过对菌株MY-5形态观察、培养特征观察、生理生化反应和16SrDNA鉴定,结果表明该菌株为链霉菌属吸水类群(Streptomyces hygroscopicus)。The aim of the study to screen anti-microorganism and find new active metabolites for control postharvest disease of fruit and vegetables. Two hundred and twenty-one actinomycetes strains were obtained from twenty-three soil samples collected from fruit orchards and vegetable fields in different parts of Guangdong Province. 9 strains showed antagonistic activities against Penicillium digitatum Sacc. Among them , strain MY-5 exhibited the strongest antibiosis capacity with an inhibitory zone of 18.67 mm. Strain MY-5 was identified as Streptomyces hygroscopicus according to its 16S rDNA sequence analysis , and the morphological , physiological and biochemical characteristics.

关 键 词：柑橘绿霉病菌 筛选 拮抗作用 放线菌 鉴定 

分 类 号：Q93[生物学—微生物学]