白细胞介素27启动子多态性与慢性乙型肝炎病毒感染的相关性研究  

Study of the Relationship between Polymorphisms of Interleukin 27 Gene in Promoter Region and Patients with Chronic Hepatitis B Virus

作　　者：祝成亮[1] 王适群[2] 张蕊[2] 刘芳[2] 汪宏良[3] 

机构地区：[1]武汉大学人民医院检验科,湖北武汉430060 [2]武汉大学生命科学学院,湖北武汉430072 [3]黄石市中心医院,湖北黄石433100

出　　处：《国际检验医学杂志》2010年第9期922-923,925,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(30970143)

摘　　要：目的探讨慢性乙型肝炎病毒感染与白细胞介素27 p28基因启动子区-964A/G多态性的关系。方法采用聚合酶链式反应限制性内切酶长度多态性(PCR-RFLP)技术对168例慢性乙型肝炎感染者和152例健康对照者白细胞介素27 p28启动子区-964A/G多态性进行分析,酶联免疫吸附试验(ELISA)检测白细胞介素27血清学水平。结果慢性乙肝感染组白细胞介素27 p28启动子区-964位点的AA基因型频率明显高于健康对照组(OR=1.75;95%CI:1.12-2.72;P=0.013);白细胞介素27水平在慢性乙肝患者显著升高(P<0.001)。结论白细胞介素27 p28启动子区-964A/G多态性与慢性乙肝病毒感染感染存在一定的相关性,A等位基因可能是HBV易感的遗传因素。Objective To explore the relationship between polymorphisms of Interleukin 27（IL-27） p28 gene in promoter region 964A/G and patients with chronic hepatitis B virus（HBV） infection. Methods IL-27p28 genotypes in promoter region 964 were analyzed among 168 patients with chronic HBV infection and 152 healthy controls by using polymerase chain reaction restriction fragment length polymorphism（PCR RFLP） , serum IL-27 levels were measured by enzyme linked immunosorbent assay （ELISA）. Results AA genotype in patients was much higher than in the healthy control group（OR= 1.75;95%CI： 1.12-2.72;P = 0. 013） ,IL-27 was significantly elevated in patients（P〈0. 001）. Conclusion The results shows that polymorphisms of IL-27p28 gene in promoter region 964 were associated with chronic HBV infeetion,A allele may be genetic factor for HBV infection.

关 键 词：肝炎病毒 乙型 基因 多态性 限制性片断长度 白细胞介素类 

分 类 号：R446.61[医药卫生—诊断学] R512.62[医药卫生—临床医学]