血管紧张素转换酶基因多态性与2型糖尿病发病的相关性研究  

Polymorphism of Angiotensin-Converting Enzyme Gene and Type 2 Diabetes

作　　者：张瑞生[1] 徐勇飞[1] 朱婵[1] 渠丽丽[1] 唐志鹏[1] 鲍倩[1] 田福亮[1] 王书奎[1] 

机构地区：[1]南京医科大学附属南京第一医院,江苏南京210006

出　　处：《现代生物医学进展》2010年第18期3486-3488,共3页Progress in Modern Biomedicine

基　　金：南京市医学科技发展项目(批准号:YKK06072)

摘　　要：目的:探讨血管紧张素转换酶基因(ACE)多态性与其血清水平及2型糖尿病(T2D)发生的相关性。方法:应用聚合酶链反应检测T2D患者287例和正常对照组307例健康人的ACE基因Alu重复序列的插入/缺失(I/D)多态性,采用全自动生化分析仪检测ACE活性及血脂水平,采用SPSS11.0软件包统计分析基因型分布和等位基因频率与其活性、血脂水平及T2D的相关性。结果:ACEI/D多态性在T2D组(DD:13.36%、ID:45.93%、II:40.72%)与对照组(DD:13.24%、ID:43.90%、II:42.86%)的基因频率无显著性差异(P>0.05)。T2D组ACE各基因型之间ACE活性有显著性差异(P<0.01)。T2D各基因型的血脂水平分析显示II型与DD型之间HDL有显著性差异(P<0.05)。结论:ACE基因DD型和D等位基因与ACE活性显著相关,但ACEI/D多态性不是T2DM发生的危险因素且无关,DD型与高HDL水平相关。Objective：To explore the association of angiotensin converting enzyme（ACE）gene polymorphism with ACE serum level and susceptibility of type 2 diabetes.Methods：287 type 2 diabetes（T2D）patients and 307 healthy individuals were enrolled in the study,and divided into T2D group and control group.The polymerase chain reaction（PCR）was applied to detect polymorphism of Alu repeated sequence insertion/deletion（I/D）in ACE gene,the activity of ACE and lipid level were detected by automatic biochemical ana-lyzer,and the SPSS10.0 software was applied to analyze the relationship between frequencies of ACE genotypes and its activity,lipid level and the occurrence of T2D.Results：There was no difference in I/D polymorphism of ACE gene between the T2D group（DD：13.36%,ID：45.93%,II：40.72%）and control group（DD：13.24%,ID：43.90%,II：42.86%）.There was significant difference of ACE activity between T2D patients with different genotypes（P0.01）.There was significant difference of HDL level between II and DD genotype in group of T2D.Conclusion：The DD genotype or D allele of ACE gene was related with its activity and the HDL level,however ACE I/D polymorphism wasn＇t the risk of the occurrence of T2D.

关 键 词：2型糖尿病 血管紧张素转化酶 插入/缺失多态性 

分 类 号：R587.1[医药卫生—内分泌]