聚丙烯腈纤维粉尘致大鼠肺部纤维化的实验研究  

Study on Fibrogenic effcts of Polyacrylonitrile Fibre Dust by Intratracheal Instillation in Rats

作　　者：张宏 张维德 王瑞 张卫东 唐宪娥 裴欣茹 李圣宝 吴捷 于锡山 蒋绪亮 付爱玲 王桂林 韩俊信 刘静 

机构地区：[1]山东省劳动卫生职业病防治研究所 [2]淄博市卫生防疫站 [3]淄博市合成纤维厂

出　　处：《劳动医学》1999年第2期79-81,共3页

摘　　要：目的：探索聚丙烯腈纤维（腈纶）粉尘能否引起肺组织纤维化。方法：取雄性大鼠７８只，随机分为实验组和对照组。实验组分二次（间隔１周）分别向气管内注入含腈纶细粒５０ｍｇ／ｍｌ和３０ｍｇ／ｍｌ的生理盐水混悬液各１ｍｌ，对照组仅注入等量生理盐水。此后饲养至３、６、１２、１８个月时，两组均分批处死，称肺干重，测全肺胶原蛋白含量，病理观察肺组织等。结果：实验组较对照组染尘后较染尘前均见肺阴影增重，密度增高，肺干重及全肺胶原蛋白含量均见明显性增加（Ｐ＜０．０５～０．０１），自染尘后１２个月起，肺间质有明显网质纤维和胶原纤维增生，以及全小叶性肺气肿改变。结论：腈纶粉尘可致肺组织纤维化，但属作用较弱的粉尘。Objectives: To explore whether polyacrylonitrile fibre dust can cause fibrosis in lung. Method: 78 male rats were randomly divided into experimental and control groups.Polyacrylonitrile dust suspension was prepared with physiological saline.The rats of experimental group were given dust suspension twice by intratracheal instillation with 1 week interval at the doses of 50mg and 30mg in 1ml,respectively.The rats of control group were only given saline in same way as mentioned above.The rats of two groups were killed at 3,6,12 and 18 months after dust instillation.The lung dry weight and collogen protein were determined,and pathohistological examination was done. Results: Lung opacity and density increased after dust instillation in experimental group,the lung dry weight and lung collogen protein obviously increased (P<0.05￣0.01).Since 12 months after dust instillation,the diffuse interstitial pulmonary fibrosis,proliferation of collogen fibres and pulmonary lobular emphysema have been observed. Conclusions: Polyacrylonitrile dust can cause lung fibrosis,but its fibrogenic effect is week.

关 键 词：聚丙烯腈 纤维粉尘 肺纤维化 

分 类 号：R135.2[医药卫生—劳动卫生] R575.202[医药卫生—公共卫生与预防医学]