大鼠肝硬化肝部分切除术后肝再生的实验研究  

HEPATOCYTES REGENERATION OF THE CIRRHOTIC LIVER AFTER PARTIAL HEPATECTOMY IN RATS

作　　者：嵇振岭[1,2] 李俊生[1,2] 严航 

机构地区：[1]南京铁道医学院附属医院普外科 [2]南京铁道医学院病理教研室

出　　处：《肝胆外科杂志》1999年第3期227-229,共3页Journal of Hepatobiliary Surgery

基　　金：铁道部科技基金

摘　　要：目的以肝硬化大鼠为动物模型，研究几种不同药物对大鼠肝脏部分切除术后肝再生的影响。方法ＳＤ大鼠１００只，６０％ＣＣＬ４石腊油混合液（０．３／１００ｇ）皮下注射，４天１次，同时饮用５％酒精溶液，经６８天制成肝硬化动物模型。模型鼠随机分为四组，１４只／组。全麻下均行２／３肝切除术，术后当天按以下方案处理：奥美拉唑组注射０．２ｍｇ／ｋｇ，１次／日；五肽胃泌素组注射０．３ｍｇ／ｋｇ，３次／日；香菇多糖组注射１ｍｇ／ｋｇ，１次／日；对照组注射生理盐水１ｍｇ／ｋｇ。连续给药一周后，每组分别随机取８只大鼠，抽取静脉血样，取下肝脏称重，并制作肝脏标本作光镜，电镜及增殖细胞核抗原（ＰＣＮＡ）检测。二周后，再对每组余下的６只大鼠行同样处理。结果奥美拉唑组及胃泌素组中，血清胃泌素水平增高，与香菇多糖组及对照组比较有统计学意义（Ｐ＜０．０５），胃泌素及奥美拉唑组的再生肝亦有明显增加（Ｐ＜０．０５），但各组间肝功能变化无明显差异。在奥美拉唑组及胃泌素组肝细胞有丝分裂指数（ＭＩ），增殖细胞核抗原（ＰＣＮＡ）阳性染色细胞量明显高于另外两组。光镜检查证实，各组肝脏均有假小叶形成，炎细胞浸润，符合肝硬化病理改变。但Ⅰ、Ⅱ组可见较多双核肝细胞及?Purpose This study is to investigate the regenerative capability of cirrhotic liver after partial hepatectomy and the possible effects of omeprazole on the hepatic regenerative process. Methods Liver cirrhosis was induced by the injection of 60% CCl4,0.3ml/100g body weight each time,repeatedly every four days.At the same time,all rats received 5% alcohol solution as the only water source.68 days later,the cirrhosis models were established.With two weeks interval after the subcutaneous injection,all cirrhotic rats were randomly separated into four groups(n=14),and each rat underwent 68% hepaectomy.The drugs were administrated on the day of the operation and lasted for one week.In Group ,administration of omeprazole 0.2mg/kg,sc.daily;Group ,pentagastrin 0.3mg/kg,sc.three times daily;Group ,lentinan,1mg.kg,sc,three times daily Group ,0.9%NS,sc.1mg/kg as control.One week after treatment,eight rats were taken out randomly from each group,and the blood was taken from the tail vein for the determination of concentration and partial hepatic function,while both the whole body weight and the remnant liver weight were determined as relative liver weight(RLW).After being killed ,the rat liver specimens were removed for light microphone and electronic microphone examination and determination of PCNA(prolifer cell nuclear antigen).Next week, the remaining six rats in each group underwent the same process. Results In the omeprazole and gastrin groups,the serum gastrin concentration was significant increased compared with lentinan and control groups(P<0.05),and the liver weight in these groups was also significant higher than that of controls (P<0.05).But there were no difference in liver function alternation.The pathology study showed that,PCNA labeling positive cells were much more higher in the omeprazole an

关 键 词：肝硬化 肝切除 肝再生 

分 类 号：R657.310.5[医药卫生—外科学]