应用RNA分析技术推断现场血痕形成时间  

Determination of Bloodstain Formation Time by RNA Analysis

作　　者：许炎[1] 蒋巍[2] 平原[1] 毕钢[1] 陈连康[1] 周怀谷[1] 

机构地区：[1]上海市公安局物证鉴定中心法医物证学现场应用技术公安部重点实验室上海市现场物证重点实验室,上海200083 [2]上海市公安局虹口分局,上海200434

出　　处：《法医学杂志》2010年第5期340-342,共3页Journal of Forensic Medicine

基　　金：上海市科学技术委员会科研计划项目(072512022)

摘　　要：目的检测血痕中β-actin mRNA和18S rRNA在人体死后8～15d期间的表达残留,为推断血痕形成时间寻找新的客观依据。方法在上述时段内每天抽提血痕总RNA,利用实时定量RT-PCR技术监测18S rRNA和β-actin mRNA的扩增状况并对产物进行定量分析,通过检测18S rRNA与β-actin mRNA含量在各个时间点的变化趋势来推测血痕形成时间。结果死后8～15 d时段内18S rRNA与β-actinmRNA量的比值呈明显升高趋势,显示出两种不同类型RNA降解的时间差异性。结论一定时段内18SrRNA与β-actin mRNA量的相对变化,可作为推测血痕形成时间的参考指标。Objective To investigate the expression of 18S rRNA and β-actin mRNA in bloodstain between 8 and 15 days after death and extrapolate the time of bloodstain formation.Methods RNA in dried bloodstain at different times was extracted,then quantified for 18S rRNA and β-actin mRNA by re-al-time RT-PCR.The bloodstain formation time was deduced based on the changes of the ratio of 18S rRNA to β-actin mRNA at different time points.Results The ratio of 18S rRNA to β-actin mRNA increased gradually with time,indicating that rRNA and mRNA degraded in different rate with time.Conclusion The ratio of 18S rRNA to β-actin mRNA could be used for estimating the time of bloodstain formation in some period.

关 键 词：法医遗传学 逆转录聚合酶链反应 血痕形成时间 

分 类 号：R331.1[医药卫生—人体生理学]