水稻抗白叶枯病基因Xa23群体的MAS育种研究  

The MAS Research in the Population of Bacterial Blight Resistance Gene Xa23 in Rice

作　　者：于洁[1] 王耀雯[1] 马文清[1] 王建岭[1] 王育荣[1] 赵开军[2] 王春连[2] 刘丕庆[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]中国农业科学院作物科学研究所,农业部作物遗传育种重点实验室,北京100081

出　　处：《华南农业大学学报》2010年第4期1-5,共5页Journal of South China Agricultural University

基　　金：“863”计划项目(2006AA10Z1B7);广西科学基金“培育含抗白叶枯病基因Xa23分子标记抗病的水稻新恢复系”(桂科回0448005);广西亚热带生物资源保护利用重点实验室、省部共建国家重点实验室培育基地开放课题(SB0502)

摘　　要：将野生稻中发现的抗白叶枯病基因Xa23转入3个优良恢复系中,为培育新的抗白叶枯病品种奠定基础.采用分子标记辅助育种(MAS)和人工剪叶接种方法快速选育抗白叶枯病新品系.选取前期所得的材料进行P6菌接种鉴定,利用Xa23基因连锁的EST标记C189对接种所得的272份接种鉴定表现有抗性的材料进行标记检测,共得到含有Xa23基因的材料254份,研究发现所得到的材料已经基本稳定,可选取最优品系参加新品种的区试.The bacterial blight resistance gene Xa23 newly found in the wild rice was introgressed into the three elite restorer lines to lay a solid foundation for the development of the bacterial blight resistance varieties.The marker-assisted selection（MAS） and leaf-cutting inoculation method were used to assay new lines.The early generation breeding materials were assayed with the inoculation of P6,and the progenies of these resistant materials were verified by the marker C189,which was closely linked with gene Xa23.Of the 272 resistant accessions revealed by the inoculation method,only 254 accessions were verified to be resistant by the marker C189 test.The late generation breeding materials were stable in phonotype,and the elite lines could be selected for the variety trial.

关 键 词：水稻 水稻白叶枯病 EST标记 Xa23基因 MAS 

分 类 号：S511[农业科学—作物学]