乙型肝炎肝硬化肝气郁结证与5-HTTLPR重复序列、TPH A218C、GNB3 C825T基因多态性相关性分析  

Association between liver cirrhosis of hepatitis B cases with Ganqi depression pattern and 5HTTLPRVNTRs,TPH A218C,GNB3 C825T polymorphism

作　　者：刘一博[1] 张玮[1] 苏式兵[2] 姜在来[2] 王磊[1] 王俐琼[1] 张海燕[1] 张丽娟[1] 

机构地区：[1]上海中医药大学附属龙华医院肝科,上海200032 [2]上海中医药大学中医复杂系统研究中心

出　　处：《中西医结合肝病杂志》2010年第5期267-271,共5页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:通过分子生物学检测,分析乙型肝炎肝硬化肝气郁结证与候选基因多态性的关联。方法:将96例乙型肝炎肝硬化患者辨证分为肝气郁结证组20例、兼肝气郁结证组24例和非肝气郁结证组52例,筛选与人体情绪调节相关的基因位点五羟色胺转运体启动子区(5-HTTLPR)重复序列、色氨酸羟化酶(TPH)A218C、G蛋白β3亚单位(GNB3)C825T位点,观察其基因多态性与肝气郁结证的相关性,为肝气郁结证的基因特征研究提供依据。结果:5-HTTLPR的S等位基因或L等位基因在各组间分布差异均无显著性意义(P>0.05),TPH A218C中肝气郁结证组患者CC基因型占45.00%(9/20)、C等位基因频率占67.50%(27/40),明显高于非肝气郁结证组的14.0%(7/50)和43.00%(43/100),差异有显著性意义(P<0.05或<0.01),而GNB3 C825T中的CC基因型和C等位基因在肝气郁结证组患者中的出现频率分别为15.00%(3/20)、42.50%(17/40),明显低于非肝气郁结证组的44.00%(22/50)和66.00%(66/100),差异有显著性意义(P<0.05)。结论:TPHA218C的CC基因型、C等位基因及GNB3 C825T T等位基因可能是乙型肝炎肝硬化肝气郁结证的易感基因,TPH A218C的A等位基因及GNB3C825T的CC基因型、C等位基因可能是其保护基因;5-HTTLPR基因多态性与该证的发生可能无相关性。Objective：To analyse the relation between liver cirrhosis of hepatitis B with Ganqi depression pattern and the candidated gene polymorphism,through the molecular biology detecton.Methods：Ninty six liver cirrhosis of hepatitis B patients were divived into group1（Ganqi depression pattern,20 cases）,group2（combining Ganqi depression pattern,24 cases）,and group3（non-Ganqi depression pattern）which contained 52 samples.Further,some gene locus which had alleles,such as 5-hydroxytryptamine transporter promoter region（5HTTLPR）,tryptophan hydroxylase（TPH）A218T,and G protein β3 subunit（GNB3）gene were screened to observe the relation between polymorphisms and Ganqi depression pattern.The results could offer objective basis for the TCM syndrome research.Results：The 5HTTLPR polymorphism had no significant difference among the three groups.TPH ＂CC＂ gene type and C alleles account for 45.0%（9/20）and 67.50%（27/40）in group1,prominently higher than 14.0%（7/50）and 43.00%（43/100）in group3（P0.05or0.01）.GNB3 ＂CC＂ gene type and C alleles account for 15.00%（3/20）and 42.50%（17/40）in group1,prominently lower than 44.00%（22/50）and 66.00%（66/100）in group3（P0.05）.Conclusion：The gene polymorphism research show that TPH A218C ＂CC＂ type,C alleles,and GNB3 C825T T alleles possibly are Ganqi depression patterns susceptible genes,while TPH A218C A alleles and GNB3 C825T ＂CC＂type,C alleles possibly are the protective gene.The 5-HTTLPR polymorphism is not related with the Ganqi depression pattern.

关 键 词：乙型肝炎肝硬化 肝气郁结证 基因多态性 五羟色胺转运体启动子区 色氨酸羟化酶A218C G蛋白β3亚单位C825) 

分 类 号：R259[医药卫生—中西医结合]