米诺环素预处理对大鼠缺血性室性心律失常的影响  

Effects of minocycline preconditioning on ischemia-induced ventricular arrhythmias in rats

作　　者：徐昌武[1] 胡笑容[1] 江洪[1] 王继春[1] 王鑫[1] 崔博[1] 鲁志兵[1] 

机构地区：[1]武汉大学人民医院心内科武汉大学心血管病研究所,湖北武汉430060

出　　处：《中国心脏起搏与心电生理杂志》2010年第5期439-441,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：目的观察米诺环素(MC)预处理对大鼠心肌缺血性室性心律失常的影响并探讨其可能机制。方法采用冠状动脉左前降支结扎法建立大鼠心肌梗死(MI)模型。将60只雄性SD大鼠随机分成6组:MI组,MI+MC组,MI+LY294002(LY)组,MI+5-羟基癸酸(5-HD)组,MI+MC+LY组及MI+MC+5-HD组。各组在制作MI模型前分别给予生理盐水、MC、LY、5-HD、MC+LY、MC+5-HD预处理。持续心电监护,观察缺血30 min内各组室性心动过速(VT)和心室颤动(VF)发生率、以及VT+VF持续时间、发生次数和室性心律失常的严重程度。缺血30min后迅速摘取心脏,用TTC法测心肌梗死面积。结果与MI组比较,MI+MC组的VT发生率无明显变化(P>0.05),但VF发生率显著降低,VT+VF持续时间、发生次数和严重程度以及心肌梗死面积显著减少(P均<0.05);而MI+MC+LY组及MI+MC+5-HD组上述指标与MI组无差异。结论 MC预处理可以减轻大鼠MI诱导的室性心律失常,这种作用可能与3-磷酸肌醇激酶/Akt信号通路和线粒体ATP敏感性钾离子通道的激活有关。Objective To investigate the effects and mechanisms of minocycline（MC） preconditioning on ischemia-induced ventricular arrhythmias in rats. Methods The model of myocardial ischemia（MI） was made by occlusion of the left anterior descending coronary artery. Sixty male Sprague-Dawley rats were randomly assigned into six groups： MI group, MI ＋ MC group, MI ＋ LY294002（LY） group, MI ＋ 5-hydroxydecanoic（5-HD） group, MI ＋ MC ＋ LY group and MI ＋ MC ＋ 5-HD group. During 30-minute ischemia, the incidence of ventricular tachycardia （VT） and ventricular fibrillation （ VF）, duration of VT ＋ VF, number of VT ＋ VF and severity of arrhythmias were monitored and analyzed. After 30-minute ischemia, the hearts were harvested and the infarct size was measured by TTC staining. Results Compared to MI group, the incidence of VF, the duration of VT ＋ VF, number of VT ＋ VF episodes, severity of ventricular arrhythmias and infarct size were significantly reduced in MI ＋ MC group（ all P 〈 0.05 ）, but not the incidence of VT（ P 〉 0.05 ）. These changes were not significant among the MI ＋ MC ＋ LY group, MI ＋ MC ＋ 5-HD group and M1 group. Conclusion Minocycline could attenuate ischemia-induced ventricular arrhythmias in rats through modulating PI3K/Akt signaling pathway and mito- chondrial KATP channels.

关 键 词：心血管病学 米诺环素 室性心律失常 心肌缺血 3-磷酸肌醇激酶 线粒体ATP敏感性钾离子通道 

分 类 号：R541.7[医药卫生—心血管疾病]