胰岛素对家兔右室心肌各层细胞动作电位的影响  

Effects of insulin on the action potential from three membranes of the rabbits′ right ventricle

作　　者：谢强[1] 张紫冠[1] 李卫华[1] 黄峥嵘[1] 王腾[2] 黄从新[2] 

机构地区：[1]厦门大学附属第一医院心内科,福建厦门361003 [2]武汉大学人民医院心内科,湖北武汉430060

出　　处：《中国心脏起搏与心电生理杂志》2010年第5期442-445,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：福建省自然科学杰出青年基金(项目编号:2009D015);福建省教育厅科技项目(项目编号:JB07104);厦门市科技局科研基金(项目编号:3502Z20094006;3502Z20089007);厦门市卫生局科研基金(项目编号:WSK0613)

摘　　要：目的探讨不同浓度胰岛素对右室三层细胞即内膜下、中膜、外膜下细胞动作电位(AP)的影响。方法采用酶解法分离右室三层心肌细胞,膜片钳技术记录右室三层细胞的AP,分别予40,280,600,2 400,4 000 pmol/L胰岛素干预后记录动作电位振幅(APA)、完成动作电位复极20%、50%、90%的时间即APD20、APD50、APD90并进行比较。结果①40 pmol/L胰岛素对右室外膜下、中膜细胞APD无明显影响;280,600,2 400,4 000 pmol/L胰岛素显著缩短右室外膜下、中膜细胞APD。②40,280,600 pmol/L胰岛素对右室内膜下细胞APD无明显影响;2 400,4000 pmol/L胰岛素显著缩短右室内膜下细胞APD。③4 000 pmol/L胰岛素使右室外膜下、中膜细胞APA辐值显著减小。结论胰岛素对右室内外膜心肌细胞的AP呈现不一致的作用,增大了跨膜动作电位梯度,可能与心律失常的发生有关。Objective To investigate the heterogeneity of action potential（AP） and the effects of different concentrations of insulin on APs of the three different membranes of rabbit＇s right ventricle. Methods The sub-epicardial（Sub-Epi） ,midcardial（ M ）, and sub-endocardial （Sub-endo） cells were obtained by enzymes digestion from right ventricle of rabbits and were intevened by different concentration of insulin at 40,280,600,2 400,4 000 pmlo/L. APs were recorded via whole-cell patch-clamp technique. Results According to current clamp protocol, APs of the three membranes of the rabbits＇ right ventricle were significantly different. （1）40 pmol/L insulin did not significantly affect AP duration（APD） of Sub-Epi and M cells ;280,600,2 400 and 4 000 pmol/L insulin significantly shortened APDs of Sub-Epi and M cells. （2）Insulin did not have significantly effect on APDs of Sub-endo ceils at the concentration of 280 and 600pmol/L;At the concentration of 2 400 and 4 000 pmol./L, insulin significantly shortened APDs of Sub-Endo. （3）4 000 pmol/L insulin significantly decreased the APA of Sub-Epi and M cells. Conclusions Insulin has different effects on APDs of the three membranes of rabbit＇s fight ventricle and may increase the voltage gradient of myocyte ,which could be the mechanisms of arrhythmia.

关 键 词：电生理学 动作电位 胰岛素 膜片钳 心肌细胞 

分 类 号：R977.15[医药卫生—药品] R331.38[医药卫生—药学]