一种新型唾液sIgA电流型纳米免疫生物传感器的制备  

Preparation of a novel salivary sIgA amperometric nano-immunosensor

作　　者：王艳[1] 甄生航[1] 郑军[1] 马霄[1] 刘成桂[1] 朱洋[1] 谢国明[1] 

机构地区：[1]重庆医科大学检验系临床检验诊断学教育部重点实验室,重庆400016

出　　处：《传感器与微系统》2010年第8期107-110,共4页Transducer and Microsystem Technologies

基　　金：重庆市科技攻关计划资助项目(CSTC2009AC5145)

摘　　要：通过层层自组装,将硫堇(Thi)和纳米金(GNPs)修饰到Nafion修饰的玻碳(GCE)电极表面,利用纳米金单层吸附唾液分泌性免疫球蛋白A(sIgA),最后用辣根过氧化物酶(HRP)封闭电极上的非特异性吸附位点,构建了一种检测唾液sIgA的新型电流型纳米免疫生物传感器。该生物传感器灵敏度高,特异性好,测试方便,检测线性范围为6.5～300 mg/L,检出限为3.0 mg/L;电流值达到95%稳态时间小于20 s。探讨了抗体和底物浓度,pH值和温度,孵育时间,干扰物对传感器的影响。该传感器与ELISA法相关性良好(R=0.98932,P<0.001),可用于唾液sIgA的快速、准确检测,从而判断人体局部免疫状况。A novel amperometric immunosensor has been developed for the detection of salivary secretory immunoglobulin A（slgA） based on layer-by-layer（LBL） assembly of gold nanoparticles-thionine （GNPs-THI） on the Nation modified glassy carbon electrode（GCE） and the slgA was adsorbed by the formative monolayer GNPs, nonspecific adsorption sites were blocked by horseradish peroxidase（HRP） eventually. This immunosensor has high sensitivity, good specificity, convenient to detect slgA with a detection limit of 3.0 mg .L^-1 , the linear range is from 6.5 to 300 mg.L^-2, the response time achieved 95 % , steady state time was less than 20 s. Some factors which influence on the biosensor were investigated, such as the concentration of antibody and substrate, pH and temperature,incubation time, interferents. To detect salivary samples, the method of biosensor and ELISA are parallel used. The results indicate that the two methods had significant correlation （ R =0. 98932, P 〈0. 001 ）, so it can be used to swiftly and accurately detect salivary sIgA and to judge the localimmunity of human body.

关 键 词：唾液SIGA 免疫生物传感器 纳米金 硫堇 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]