生化汤谱效关系初探  

Primary investigation on chromatogram-pharmacodynamics relationship of shenghua decoction

作　　者：陈永刚[1] 刘晓[1] 汤道权[1] 印晓星[1] 时晓东[2] 常艳华[1] 杜倩[1] 

机构地区：[1]徐州医学院药学院,江苏徐州221002 [2]徐州市第一人民医院检验科,江苏徐州221002

出　　处：《成都医学院学报》2010年第2期126-131,共6页Journal of Chengdu Medical College

基　　金：江苏省高校自然科学基金(08KJB360010)

摘　　要：目的研究生化汤不同配伍组方的药效与其化学成分指纹图谱间的关系,阐述生化汤配伍机理。方法按照君臣佐使中医组方原理,采用撤药分析法对生化汤进行拆方研究,观察比较各配伍组方对急性血瘀大鼠血液流变学和血浆血栓素B2(TXB2)、前列环素F1α(PGF1α)的药效作用的不同,并从整体上考察各配伍组方化学成分指纹图谱间的差异。结果缺桃仁组方对急性血瘀大鼠的全血黏度、血浆黏度、TXB2水平和TXB2/PGF1α的值的影响与生化汤全方比较均有显著性差异(P<0.05或P<0.01);缺川芎组方对红细胞聚集指数、红细胞压积、红细胞电泳指数、血沉方程K值的影响与全方比较亦有显著性差异(P<0.05或P<0.01)。结论生化汤全方对急性血瘀大鼠的治疗作用优于其他缺药组方,而上述13种物质均有可能是生化汤药效物质基础的重要组成部分。Objective To explore the relationship between shenghua decoction＇s pharmacological effects and HPLC fingerprint within five different compositions and to clarify the compatibility principles of the prescription. Methods Following the traditional Chinese medical （TCM） theory of principal,assistant,complement and mediating guide and dismantling drugs to determine the compatibility regularity of medicinal materials,the effects of different shenghua decoction compositions on hemorheology,thromboxane B2（TXB2）,and prostaglandin F1α（PGF1α） in acute blood stasis model of rats were observed; meanwhile,the fingerprint spectrum changes between the compositions were investigated as a whole. Results Two prescriptions without Prunus persica,compared with original shenghua decoction,had significant effect on plasma viscosity,blood viscosity,contents of TXB2 and TXB2/PGF1α （P〈0.05 or P〈0.01）; the prescription without Ligusticum chuanxiong,compared with original shenghua decoction,significantly increased volume of packed red blood cell,blood cell aggregation index,erythrocyte electrophoretic index,and K value of blood sedimentation equation （P〈0.05 or P〈0.01）.Conclusion The original shenghua decoction has better therapeutic effect on acute blood stasis model of rats than other compati-bility groups missing herbs.The 13 components mentioned may be the indispensable parts of material basis of TCM preparation of shenghua decoction.

关 键 词：生化汤 撤药分析 谱效关系 物质基础 

分 类 号：R285.5[医药卫生—中药学]